Paper Report for: Ensing_1984_Pharm.Weekbl.Sci_6_241
Reference
Title: Does the tissue concentration in receptor binding studies change the affinity of the labelled ligand? Ensing K, de Zeeuw RA Ref: Pharm Weekbl Sci, 6:241, 1984 : PubMed
When the tissue concentration in a radioreceptor assay for anticholinergic drugs was varied in order to obtain optimum conditions, and the receptor concentration Cr and the equilibrium dissociation constant KD were determined by Scatchard analysis, the KD increased with increasing tissue concentrations. This phenomenon was considered as an artefact caused by non-specific binding of the labelled ligand to constituents of the receptor preparation which were not completely retained on the glass-fibre filters used for the separation of bound and free fraction of radio-labelled ligand. The increase in KD in these experiments could be described with a mathematical model of the binding experiments.
        
Related information
Citations formats
Ensing K, de Zeeuw RA (1984) Does the tissue concentration in receptor binding studies change the affinity of the labelled ligand? Pharm Weekbl Sci6: 241-4
Ensing K, de Zeeuw RA (1984) Pharm Weekbl Sci6: 241-4