The new simple technique of rectal mucosal punch biopsy has been used successfully in 170 cases for diagnosing Hirschsprung's disease. The presented punch biopsy method, using a test tube with a side hole and laryngeal biopsy forceps, has proven to be superior to the rectal suction biopsy method associated with several demerits.
        
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Hirose R, Hirata Y, Yamada T, Kawana T, Taguchi T, Suita S (1993) The simple technique of rectal mucosal biopsy for the diagnosis of Hirschsprung's disease Journal of Pediatric Surgery28: 942-4
Hirose R, Hirata Y, Yamada T, Kawana T, Taguchi T, Suita S (1993) Journal of Pediatric Surgery28: 942-4