cDNAs encoding rat adipose tissue hormone-sensitive lipase were expressed in COS cells, under the control of the SV40 promoter to half the level in rat adipocytes, the richest native source of the enzyme. A cDNA lacking most of the long 5'-untranslated region of the full-length rat hormone-sensitive lipase cDNA was, with regard to the lipase activity, on the average 70% more efficiently expressed that the full-length cDNA. The recombinant protein was almost identical to hormone-sensitive lipase of rat adipose tissue with respect to specific activity, susceptibility to inhibitors, molecular size, phosphorylation and activation by cyclic AMP-dependent protein kinase. The described eukaryotic expression system will allow analysis of effects of amino acid substitutions introduced into the lipase molecule by site-directed mutagenesis.
        
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Holm C, Davis RC, Fredrikson G, Belfrage P, Schotz MC (1991) Expression of biologically active hormone-sensitive lipase in mammalian (COS) cells FEBS Letters285: 139-44
Holm C, Davis RC, Fredrikson G, Belfrage P, Schotz MC (1991) FEBS Letters285: 139-44