Paper Report for: Huang_2020_Angew.Chem.Int.Ed.Engl_59_4349
Reference
Title: Collaborative Biosynthesis of a Class of Bioactive Azaphilones by Two Separate Gene Clusters Containing Four PKS/NRPSs with Transcriptional Crosstalk in Fungi Huang X, Zhang W, Tang S, Wei S, Lu X Ref: Angew Chem Int Ed Engl, 59:4349, 2020 : PubMed
Azaphilones are a family of fungal polyketide metabolites with diverse chemical structures and biological activities with a highly oxygenated pyranoquinone bicyclic core. Here, a class of azaphilones possessing a 6/6/6/6 tetracyclic ring system was identified in Aspergillus terreus, and exhibited potential anticancer activities. The gene deletions and biochemical investigations demonstrated that these azaphilones were collaboratively synthesized by two separate clusters containing four core-enzymes, two nonreducing PKSs, one highly reducing PKS, and one NRPS-like. More interestingly, we found that the biosynthesis is coordinately regulated by a crosstalk mechanism between these two gene clusters based on three transcriptional factors. This is a meaningful mechanism of fungal secondary metabolism, which allows fungi to synthesize more complex compounds and gain new physiological functions. The results provide a new insight into fungal natural product biosynthesis.
Huang X, Zhang W, Tang S, Wei S, Lu X (2020) Collaborative Biosynthesis of a Class of Bioactive Azaphilones by Two Separate Gene Clusters Containing Four PKS/NRPSs with Transcriptional Crosstalk in Fungi Angew Chem Int Ed Engl59: 4349-4353
Huang X, Zhang W, Tang S, Wei S, Lu X (2020) Angew Chem Int Ed Engl59: 4349-4353