Title: Single-cell-resolved measurement of enzyme activity at the tissue level using drop-on-demand microkits Huang R, Jin R, Jiang D, Chen HY Ref: Analyst, :, 2021 : PubMed
Drop-on-demand microkits with a diameter of ~20 microm are used to measure the activity of acetylcholinesterase (AChE) in a brain slice with single-cell resolution. The relative standard deviation from 25 cellular regions reached 73.3% exhibiting the difference of enzyme activity in the brain slice. Therefore, this approach utilizing the well-established kits provides an alternative single-cell-resolved strategy for the elucidation of enzymatic heterogeneity at the tissue level.
        
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Huang R, Jin R, Jiang D, Chen HY (2021) Single-cell-resolved measurement of enzyme activity at the tissue level using drop-on-demand microkits Analyst