Title: Identification and Study of Biomarkers from Novichok-Inhibited Butyrylcholinesterase in Human Plasma Jeong WH, Lee JY, Lim KC, Kim HS Ref: Molecules, 26:, 2021 : PubMed
To identify biomarkers of ethyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A234)- or methyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A232)-inhibited butyrylcholinesterase (BChE), we investigated nonapeptide adducts containing the active site serine, which plays a key role in enzyme activity, using LC-MS/HRMS. Biomarkers were acquired as expected, and they exhibited a significant amount of fragment ions from the inhibiting agent itself, in contrast to the MS2 spectra of conventional nerve agents. These biomarkers had a higher abundance of [M+2H](2+) ions than [M+H](+) ions, making doubly charged ions more suitable for trace analysis.
Jeong WH, Lee JY, Lim KC, Kim HS (2021) Identification and Study of Biomarkers from Novichok-Inhibited Butyrylcholinesterase in Human Plasma Molecules26:
Jeong WH, Lee JY, Lim KC, Kim HS (2021) Molecules26: