Paper Report for: Jiang_2009_Biochem.Biophys.Res.Commun_378_269
Reference
Title: Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) Jiang X, Qu M, Denholm I, Fang J, Jiang W, Han Z Ref: Biochemical & Biophysical Research Communications, 378:269, 2009 : PubMed
Two full-length genes encoding different acetylcholinesterases (AChEs), designated as Ch-ace1 and Ch-ace2, were cloned from strains of the rice stem borer (Chilo suppressalis) susceptible and resistant to the organophosphate insecticide triazophos. Sequence analysis found an amino acid mutation A314S in Ch-ace1 (corresponding to A201 in Torpedo californica AChE) that was consistently associated with the occurrence of resistance. This mutation removed an MspA1 I restriction site from the wild type allele. An assay based on restriction fragment length polymorphism (RFLP) analysis was developed to diagnose A314S genotypes in field populations. Results showed a strong correlation between frequencies of the mutation and phenotypic levels of resistance to triazophos. The assay offers a prospect for rapid monitoring of resistance and assisting with the appropriate choice of insecticide for combating damage caused by C. suppressalis.
Jiang X, Qu M, Denholm I, Fang J, Jiang W, Han Z (2009) Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) Biochemical & Biophysical Research Communications378: 269-72
Jiang X, Qu M, Denholm I, Fang J, Jiang W, Han Z (2009) Biochemical & Biophysical Research Communications378: 269-72