Paper Report for: Ju_2013_Bioresour.Technol_128_81
Reference
Title: Characterization and preparation of highly stable aggregates of a novel type of hydrolase (BL28) from Bacillus licheniformis Ju H, Jang E, Ryu BH, Kim TD Ref: Bioresour Technol, 128:81, 2013 : PubMed
A novel type of hydrolase (BL28) from Bacillus licheniformis was identified, expressed in Escherichia coli, characterized, and immobilized for industrial applications. Biochemical characteristics of BL28 were investigated by performing SDS-PAGE, mass spectrometry, enzyme assays, CD spectroscopy, intrinsic fluorescence, and in silico analysis. Furthermore, cross-linked enzyme aggregates (CLEAs) of BL28 were prepared. These CLEA-BL28 aggregates exhibited improved catalytic efficiencies and stabilities compared to free BL28 against harsh conditions of thermal or chemical stress as well as high reusability. The characteristics of the CLEA-BL28 aggregates highlight their great potentials in pharmaceutical and chemical industries.
Ju H, Jang E, Ryu BH, Kim TD (2013) Characterization and preparation of highly stable aggregates of a novel type of hydrolase (BL28) from Bacillus licheniformis Bioresour Technol128: 81-6
Ju H, Jang E, Ryu BH, Kim TD (2013) Bioresour Technol128: 81-6