Paper Report for: Ke_2021_Ecotoxicol.Environ.Saf_224_112666
Reference
Title: Heterologous expression and exploration of the enzymatic properties of the carbaryl hydrolase CarH from a newly isolated carbaryl-degrading strain Ke Z, Zhu Q, Jiang W, Zhou Y, Zhang M, Jiang M, Hong Q Ref: Ecotoxicology & Environmental Safety, 224:112666, 2021 : PubMed
Carbaryl is the representative of carbamate insecticide. As an acetylcholinesterase inhibitor, it poses potential threat to humans and other non-target organisms. Agrobacterium sp. XWY-2, which could grow with carbaryl as the sole carbon source, was isolated and characterized. The carH gene, encoding a carbaryl hydrolase, was cloned from strain XWY-2 and expressed in Escherichia coli BL21 (DE3). CarH was able to hydrolyze carbamate pesticides including carbaryl, carbofuran, isoprocarb, propoxur and fenobucarb efficiently, while it hydrolyzed oxamyl and aldicarb poorly. The optimal pH of CarH was 8.0 and the optimal temperature was 30 degC. The apparent K(m) and k(cat) values of CarH for carbaryl were 38.01 +/- 2.81 microM and 0.33 +/- 0.01 s(-1), respectively. The point mutation experiment demonstrated that His341, His343, His346, His416 and D437 are the key sites for CarH to hydrolyze carbaryl.
        
Comment
not Alpha/beta hydrolase but beta lacamase
Related information
Citations formats
Ke Z, Zhu Q, Jiang W, Zhou Y, Zhang M, Jiang M, Hong Q (2021) Heterologous expression and exploration of the enzymatic properties of the carbaryl hydrolase CarH from a newly isolated carbaryl-degrading strain Ecotoxicology & Environmental Safety224: 112666
Ke Z, Zhu Q, Jiang W, Zhou Y, Zhang M, Jiang M, Hong Q (2021) Ecotoxicology & Environmental Safety224: 112666