Paper Report for: Kelle_2016_Biotechnol.Appl.Biochem_63_852
Reference
Title: Heterologous production of a feruloyl esterase from Pleurotus sapidus synthesizing feruloyl-saccharide esters Kelle S, Nieter A, Krings U, Zelena K, Linke D, Berger RG Ref: Biotechnol Appl Biochem, 63:852, 2016 : PubMed
The feruloyl esterase (FAE) gene EST1 from the basidiomycete Pleurotus sapidus was heterologously expressed in Escherichia coli and Pichia pastoris. Catalytically active recombinant Est1 was secreted using P. pastoris as a host. For expression in P. pastoris, the expression vector pPIC9K was applied. The EST1 gene was cloned with an N-terminal alpha-mating factor pre-pro sequence and expressed under the control of a methanol inducible alcohol oxidase 1 promotor. Est1 was purified to homogeneity using ion exchange and hydrophobic interaction chromatography. The recombinant Est1 showed optima at pH 5.0 and 50 degrees C, and released ferulic acid from saccharide esters and from the natural substrate destarched wheat bran. Substrate specificity profile and descriptor-based analysis demonstrated unique properties, showing that Est1 did not fit into the current FAE classification model. Transferuloylation synthesis of feruloyl-saccharide esters was proven for mono- and disaccharides.
Kelle S, Nieter A, Krings U, Zelena K, Linke D, Berger RG (2016) Heterologous production of a feruloyl esterase from Pleurotus sapidus synthesizing feruloyl-saccharide esters Biotechnol Appl Biochem63: 852-862
Kelle S, Nieter A, Krings U, Zelena K, Linke D, Berger RG (2016) Biotechnol Appl Biochem63: 852-862