The four kinds of subunits of the Torpedo californica nicotinic acetylcholine receptor have been produced in various combinations by injecting Xenopus oocytes with the corresponding subunit-specific mRNAs synthesized by transcription in vitro of the cloned cDNAs. Functional analysis suggests that association of the alpha-subunit with either the gamma- or the delta-subunit is a prerequisite for generating the conformation necessary for agonist binding. The acetylcholine receptor devoid of either the beta-, gamma- or delta-subunit exhibits weak channel activity.
        
Related information
Citations formats
Kurosaki T, Fukuda K, Konno T, Mori Y, Tanaka K, Mishina M, Numa S (1987) Functional properties of nicotinic acetylcholine receptor subunits expressed in various combinations FEBS Letters214: 253-8
Kurosaki T, Fukuda K, Konno T, Mori Y, Tanaka K, Mishina M, Numa S (1987) FEBS Letters214: 253-8