Paper Report for: Li_2009_J.Chromatogr.A_1216_2071
Reference
Title: Quality evaluation of Rhizoma Belamcandae (Belamcanda chinensis (L.) DC.) by using high-performance liquid chromatography coupled with diode array detector and mass spectrometry Li J, Li WZ, Huang W, Cheung AW, Bi CWC, Duan R, Guo AJ, Dong TTX, Tsim KWK Ref: Journal of Chromatography A, 1216:2071, 2009 : PubMed
A high-performance liquid chromatography coupled with diode array detector and mass spectrometry (HPLC-DAD-MS) method was developed to evaluate the quality of Rhizoma Belamcandae (Belamcanda chinensis (L.) DC.) through establishing chromatographic fingerprint and simultaneous determination of seven phenolic compounds. The analysis was achieved on an Alltima C(18) analytical column (250 mm x 4.6 mm i.d. 5 microm) using linear gradient elution of acetonitrile-0.1% trifluoroacetic acid. The correlation coefficients of similarity were determined from the HPLC fingerprints, and they shared a close similarity. By using an online APCI-MS/MS, twenty phenols were identified. In addition, seven of these phenols including mangiferin, 7-O-methylmangiferin, tectoridin, resveratrol, tectorigenin, irigenin and irisflorentin were quantified by the validated HPLC-DAD method. These phenols are considered to be major constituents in Rhizoma Belamcandae, and are generally regarded as the index for quality assessment of this herb. This developed method by having a combination of chromatographic fingerprint and quantification analysis could be applied to the quality control of Rhizoma Belamcandae.
        
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Citations formats
Li J, Li WZ, Huang W, Cheung AW, Bi CWC, Duan R, Guo AJ, Dong TTX, Tsim KWK (2009) Quality evaluation of Rhizoma Belamcandae (Belamcanda chinensis (L.) DC.) by using high-performance liquid chromatography coupled with diode array detector and mass spectrometry Journal of Chromatography A1216: 2071-8
Li J, Li WZ, Huang W, Cheung AW, Bi CWC, Duan R, Guo AJ, Dong TTX, Tsim KWK (2009) Journal of Chromatography A1216: 2071-8