We assessed the integrity of cholinergic neurotransmission in parietal cortex of young adult (7 months) and aged (17 months) transgenic APPswe/PS1dE9 female mice compared to littermate controls. Choline acetyltransferase and acetylcholinesterase activity declined age-dependently in both genotypes, whereas both age- and genotype-dependent decline was found in butyrylcholinesterase activity, vesicular acetylcholine transporter density, muscarinic receptors and carbachol stimulated binding of GTP gamma S in membranes as a functional indicator of muscarinic receptor coupling to G-proteins. Notably, vesicular acetylcholine transporter levels and muscarinic receptor-G-protein coupling were impaired in transgenic mice already at the age of 7 months compared to wild type littermates. Thus, brain amyloid accumulation in this mouse model is accompanied by a serious deterioration of muscarinic transmission already before the mice manifest significant cognitive deficits.
        
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Machova E, Jakubik J, Michal P, Oksman M, Iivonen H, Tanila H, Dolezal V (2008) Impairment of muscarinic transmission in transgenic APPswe/PS1dE9 mice Neurobiology of Aging29: 368-78
Machova E, Jakubik J, Michal P, Oksman M, Iivonen H, Tanila H, Dolezal V (2008) Neurobiology of Aging29: 368-78