Paper Report for: Miyamoto_2010_Biochem.Biophys.Res.Commun_394_972
Reference
Title: The creation of the artificial RING finger from the cross-brace zinc finger by alpha-helical region substitution Miyamoto K, Togiya K Ref: Biochemical & Biophysical Research Communications, 394:972, 2010 : PubMed
The creation of the artificial RING finger as ubiquitin-ligating enzyme (E3) has been demonstrated. In this study, by the alpha-helical region substitution between the EL5 RING finger and the Williams-Beuren syndrome transcription factor (WSTF) PHD finger, the artificial E3 (WSTF PHD_RING finger) was newly created. The experiments of the chemical modification of residues Cys and the circular dichroism spectra revealed that the WSTF PHD_RING finger binds two zinc atoms and adopts the zinc-dependent ordered-structure. In the substrate-independent ubiquitination assay, the WSTF PHD_RING finger functions as E3 and was poly- or mono-ubiquitinated. The present strategy is very simple and convenient, and consequently it might be widely applicable to the creation of various artificial E3 RING fingers with the specific ubiquitin-conjugating enzyme (E2)-binding capability.
        
Related information
Citations formats
Miyamoto K, Togiya K (2010) The creation of the artificial RING finger from the cross-brace zinc finger by alpha-helical region substitution Biochemical & Biophysical Research Communications394: 972-5
Miyamoto K, Togiya K (2010) Biochemical & Biophysical Research Communications394: 972-5