Paper Report for: Mokrushina_2016_Bull.Exp.Biol.Med_161_83
Reference
Title: New Genetic Constructs for Generation of Stable Therapeutic Antibodies to Organophosphorus Toxins in Methylotrophic Yeasts Pichia Pastoris Mokrushina YA, Stepanova AV, Bobik TV, Smirnov IV, Gabibov AG Ref: Bulletin of Experimental Biology & Medicine, 161:83, 2016 : PubMed
We propose a new method of obtaining of stable Fab-fragments of antibodies in Pichia pastoris expression system. Recently, we obtained Fab-fragments of antibodies neutralizing organophosphorus toxins. However, high yield of the target products was not attained because of high level of proteolytic degradation. In the present study, we identified sites of proteolytic degradation in Fab-fragments and endogenous proteases performing degradation, which allowed obtaining optimized genetic constructs for expression of antibody heavy chains (IgGgamma1) and kappa and lambda isotypes of light chains. Co-transformation of these vectors allowed obtaining Fab-fragments of antibodies to organophosphorus toxins without proteolytic degradation of the product.
        
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Mokrushina YA, Stepanova AV, Bobik TV, Smirnov IV, Gabibov AG (2016) New Genetic Constructs for Generation of Stable Therapeutic Antibodies to Organophosphorus Toxins in Methylotrophic Yeasts Pichia Pastoris Bulletin of Experimental Biology & Medicine161: 83-7
Mokrushina YA, Stepanova AV, Bobik TV, Smirnov IV, Gabibov AG (2016) Bulletin of Experimental Biology & Medicine161: 83-7