Paper Report for: Mukherjee_2015_Int.J.Biol.Macromol_81_544
Reference
Title: Molecular bioimprinting of lipases with surfactants and its functional consequences in low water media Mukherjee J, Gupta MN Ref: Int J Biol Macromol, 81:544, 2015 : PubMed
Lipases from Thermomyces lanuginosa (TLL), Candida rugosa (CRL) and Burkholderia cepacia (BCL) were obtained in the 'open lid' form by adding surfactant molecules like n-octyl-beta-d-glucopyranoside (OG), hexadecyl trimethyl ammonium bromide (CTAB), Bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT) and triton X-100 for this purpose. The enzymes were 'dried' by precipitating with 4x (v/v) excess of organic solvents. The imprint surfactant molecules were removed by extensive washing with organic solvents. TLL imprinted with 0.05% CTAB showed 11-fold increase in the transesterification activity and was a better preparation to kinetically resolve (+/-)-1-phenylethanol. Fluorescence emission spectra confirmed that Trp89 of the lid was indeed affected during bioimprinting. With CRL, bioimprinting with OG gave 7-fold increase in the transesterification rates and resulted in reversal of enantioselectivity of CRL and gave R-phenylethyl acetate instead of the S-product as with the unimprinted precipitate. Bioimprinted BCL was also a 7-fold better catalyst for transesterification as well as enantioselectivity.
        
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Mukherjee J, Gupta MN (2015) Molecular bioimprinting of lipases with surfactants and its functional consequences in low water media Int J Biol Macromol81: 544-51
Mukherjee J, Gupta MN (2015) Int J Biol Macromol81: 544-51