Title: The influence of cholinergic agents on histamine release from HMC-1 human mast cell line stimulated with IgG, C-reactive protein and compound 48/80 Nazarov PG, Pronina AP Ref: Life Sciences, 91:1053, 2012 : PubMed
AIMS: The aim of this work is to study the role of acetylcholine (ACh) receptors (AChRs) in the regulation of FcgammaR activity in human mast cells (MC) activated by aggregated IgG (aIgG) and CRP. MC, the key regulators at the interface of innate and acquired immunity, have abundant Fc receptors for IgG (FcgammaRII) which indicate the role of their ligands, IgG and C-reactive protein (CRP), in regulating MC activity. Cholinergic control of FcgammaR-dependent MC functions is poorly defined. MAIN METHODS: HMC-1 culture of human MC; cell incubations with cholinergic drugs and FcgammaR ligands such as heat aggregated human IgG or purified human CRP; compound 48/80, a known histamine liberator employing G protein-coupled receptors, was used as a positive control of MC degranulation; assessment of histamine release. KEY FINDINGS: Both nAChR and mAChR antagonists (hexamethonium and methacine, respectively), per se, elevated histamine-releasing activity of the HMC-1 and suppressed the MC responses to most of investigated activators (carbachol, compound 48/80, and to a lesser extent aIgG). Two blockers together should be applied to aIgG-stimulated cells in order to obtain appreciable suppression of histamine release. The FcgammaR-mediated HMC-1 cell response to CRP was the least sensitive to attenuation by ACh signaling. SIGNIFICANCE: The data obtained suggest the involvement of ACh in the functioning of other receptor systems. Our results indicate that AChRs are closely associated with G protein-coupled receptor-induced reactions of MC and optionally with FcgammaR-dependent functions. CONCLUSION: The data presented demonstrate that AChRs and endogenous ACh are involved in regulating mast cell degranulation and histamine release by affecting the functions of receptors to compound 48/80 and, less, FcgammaRs.
        
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Nazarov PG, Pronina AP (2012) The influence of cholinergic agents on histamine release from HMC-1 human mast cell line stimulated with IgG, C-reactive protein and compound 48/80 Life Sciences91: 1053-7
Nazarov PG, Pronina AP (2012) Life Sciences91: 1053-7