Title: Release of LHRH is linearly related to the time integral of presynaptic Ca2+ elevation above a threshold level in bullfrog sympathetic ganglia Peng YY, Zucker RS Ref: Neuron, 10:465, 1993 : PubMed
To study the Ca2+ dependency of luteinizing hormone-releasing hormone (LHRH) release in the bullfrog sympathetic ganglia, a method was developed to fill the preganglionic nerve terminal boutons with membrane-impermeant fura-2. We found that as stimulation frequency increased from 0.5 to 40 Hz, the peak [Ca2+]i ([Ca2+]p) and the rate of rise in [Ca2+]i increased, the decay of [Ca2+]i transients followed up to three exponentials, and release of LHRH was linearly related to integral of ([Ca2+]i--[Ca2+]t)dt. The threshold level of [Ca2+]i for LHRH release for a given set of boutons on a C cell, [Ca2+]t, was estimated by the [Ca2+]p evoked by 0.5 Hz stimulation that does not induce LHRH release.
        
Related information
Citations formats
Peng YY, Zucker RS (1993) Release of LHRH is linearly related to the time integral of presynaptic Ca2+ elevation above a threshold level in bullfrog sympathetic ganglia Neuron10: 465-73