Rapid kinetic studies of histrionicotoxin interactions with membrane-bound acetylcholine-receptor showed a conformational change in the receptor-histironicotoxin complex as reflected by a decrease in fluorescence intensity of the extrinsic probe ethidium. The simplest kinetic mechanism consistent with the observed data is one in which a rapid preequiliibrium exists between receptor and toxin (K = 3.33 micrometers), followed by a slow conformational change (k1 congruent to 2 X 10(-2) s-1 and k-1 congruent to 1.5 X 10(-3) s-1). The overall equilibrium constant (Kov) determined from a fit of the amplitude dependence on toxin concentration had a value of 0.25 micrometer. The data preclude kinetic mechanisms where histrionicotoxin acts as an effector, shifting equilibria between preexisting, discrete, and slowly interconverting receptor forms.
        
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Schimerlik MI, Quast U, Raftery MA (1979) Ligand-induced changes in membrane-bound acetylcholine receptor observed by ethidium fluorescence. 3. Stopped-flow studies with histrionicotoxin Biochemistry18: 1902-6
Schimerlik MI, Quast U, Raftery MA (1979) Biochemistry18: 1902-6