Paper Report for: Schreck_2009_Ecotoxicol.Environ.Saf_72_1609
Reference
Title: ChE, GST and CAT: evaluation of the efficiency of a combined buffer for protein extraction Schreck E, Geret F, Gontier L, Treilhou M Ref: Ecotoxicology & Environmental Safety, 72:1609, 2009 : PubMed
Toxicity and biotransformation of several earthworm contaminants are widely evaluated nowadays using biochemical biomarkers. Many investigations track enzyme activities as biomarkers of neurotoxicity (cholinesterase (ChE)), metabolisation (glutathione-S-transferase (GST)) and oxidative stress (catalase (CAT)). This study proposes an evaluation of the use of a combined buffer, to extract proteins from earthworms and then analyse the 3 biomarkers. The method provides good results and allows protein extraction and quantitative determination of biomarkers with the same efficiency as the enzyme-specific buffers. It decreases preparation time and permits a study of the biomarkers on the same individual with only one homogenisation.
        
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Citations formats
Schreck E, Geret F, Gontier L, Treilhou M (2009) ChE, GST and CAT: evaluation of the efficiency of a combined buffer for protein extraction Ecotoxicology & Environmental Safety72: 1609-13
Schreck E, Geret F, Gontier L, Treilhou M (2009) Ecotoxicology & Environmental Safety72: 1609-13