Crude membranes (27,000 x g pellets) from three normal human pancreata were prepared. Muscarinic receptors were investigated by the ability of three antagonists (atropine, pirenzepine, and AF-DX 116) and three agonists (carbamylcholine, oxotremorine, and pilocarpine) to inhibit [3H]NMS binding. These receptors showed for pirenzepine and AF-DX 116 a M2 beta specificity, typical of secretory glands and smooth muscle, that was comparable to that of rat pancreatic membranes, i.e., a low affinity for the two antagonists (Ki of 0.4 and 0.2 microM, respectively). In addition, these receptors were predominantly in a low affinity state for the agonist carbamylcholine (Ki of 100 microM).
        
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Waelbroeck M, Camus J, Tastenoy M, de Neef P, Scemama JL, Fourmy D, Vaysse N, Pradayrol L, Robberecht P, Christophe J (1988) Characterization of muscarinic receptors in human pancreatic membranes Pancreas3: 627-30
Waelbroeck M, Camus J, Tastenoy M, de Neef P, Scemama JL, Fourmy D, Vaysse N, Pradayrol L, Robberecht P, Christophe J (1988) Pancreas3: 627-30