Title: HIF-1alpha up-regulates NDRG1 expression through binding to NDRG1 promoter, leading to proliferation of lung cancer A549 cells Wang Q, Li LH, Gao GD, Wang G, Qu L, Li JG, Wang CM Ref: Mol Biol Rep, 40:3723, 2013 : PubMed
Hypoxia-inducible signaling pathway is involved in many pathological processes, such as adaptiveness regulation of plateau environment, myocardial ischemia and tumorigenesis. NDRG1 is a member of the N-myc downregulated gene (NDRG) family, and it has strong hypoxia stress reaction functions. Although the cellular responses to hypoxia are well known, little is known about the interaction between hypoxia-inducible transcription factor (HIF)-1alpha and NDRG1. In this study, we cloned HIF-1alpha CDS, NDRG1 promoter and its truncatures, constructed pCDNA3.0-Hif-1alpha and pGL3-basic-NDRG1. Reporter assay results showed that HIF-1alpha could bind to NDRG1 promoter to activate NDRG1 expression. Further results revealed that -1202 to -450 of NDRG1 promoter is the most important region for HIF-1alpha binding. Then, we constructed NDRG1 stable transfection cell line. Results from MTT, colony-forming assay and flow cytometry showed that NDRG1 overexpression results in more proliferation and less apoptosis of A549 lung cancer cells. Our study elucidates the mechanism of NGRG1 in hypoxia stress reactions and may provide new strategy for hypoxia injuries.
        
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Wang Q, Li LH, Gao GD, Wang G, Qu L, Li JG, Wang CM (2013) HIF-1alpha up-regulates NDRG1 expression through binding to NDRG1 promoter, leading to proliferation of lung cancer A549 cells Mol Biol Rep40: 3723-9
Wang Q, Li LH, Gao GD, Wang G, Qu L, Li JG, Wang CM (2013) Mol Biol Rep40: 3723-9