Paper Report for: Weston_2009_Environ.Sci.Technol_43_5514
Reference
Title: Use of engineered enzymes to identify organophosphate and pyrethroid-related toxicity in toxicity identification evaluations Weston DP, Jackson CJ Ref: Environ Sci Technol, 43:5514, 2009 : PubMed
Engineered variants of a carboxylesterase from Lucilia cuprina (E3) and a phosphotriesterase from Agrobacterium radiobacter (OpdA) with enhanced hydrolytic activities against pyrethroid and organophosphate pesticides were evaluated as a toxicity identification evaluation (TIE) manipulation. Reduction in toxicity in the presence of the enzyme provides an indication that the toxicant is the enzyme's target substrate. Carboxy/esterase E3 variants were evaluated to determine if the enzymes could mitigate toxicity of pyrethroids to the amphipod, Hyalella azteca. Enzymes were able to achieve 12-70-fold reduction in toxicity for bifenthrin, cyfluthrin, and cypermethrin in water. Only a 2-fold reduction in toxicity was observed with pyrethroid-contaminated sediment though the phosphotriesterase OpdA achieved at least a 35-fold reduction in toxicity from the organophosphate chlorpyrifos in sediment. Tests with urban runoff samples and agriculture-affected sediments demonstrated that the enzymes could be useful in TIEs to identify pesticide-related toxicity. The approach promises to be a useful TIE tool for organophosphate and pyrethroid pesticides, particularly in a water matrix, and potentially could be used for identification of toxicity attributable to other pesticides.
        
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Citations formats
Weston DP, Jackson CJ (2009) Use of engineered enzymes to identify organophosphate and pyrethroid-related toxicity in toxicity identification evaluations Environ Sci Technol43: 5514-20
Weston DP, Jackson CJ (2009) Environ Sci Technol43: 5514-20