Paper Report for: Wu_2013_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_69_456
Reference
Title: Expression, purification, crystallization and preliminary X-ray analysis of tannase from Lactobacillus plantarum. Wu M, Peng X, Wen H, Wang Q, Chen Q, McKinstry WJ, Ren B Ref: Acta Crystallographica Sect F Struct Biol Cryst Commun, 69:456, 2013 : PubMed
Tannase catalyses the hydrolysis of the galloyl ester bond of tannins to release gallic acid. It belongs to the serine esterases and has wide applications in the food, feed, beverage, pharmaceutical and chemical industries. The tannase from Lactobacillus plantarum was cloned, expressed and purified. The protein was crystallized by the sitting-drop vapour-diffusion method with microseeding. The crystals belonged to space group P1, with unit-cell paramters a = 46.5, b = 62.8, c = 83.8 A, alpha = 70.4, beta = 86.0, gamma = 79.4degre. Although the enzyme exists mainly as a monomer in solution, it forms a dimer in the asymmetric unit of the crystal. The crystals diffracted to beyond 1.60A resolution using synchrotron radiation and a complete data set was collected to 1.65A resolution.
Wu M, Peng X, Wen H, Wang Q, Chen Q, McKinstry WJ, Ren B (2013) Expression, purification, crystallization and preliminary X-ray analysis of tannase from Lactobacillus plantarum. Acta Crystallographica Sect F Struct Biol Cryst Commun69: 456-9
Wu M, Peng X, Wen H, Wang Q, Chen Q, McKinstry WJ, Ren B (2013) Acta Crystallographica Sect F Struct Biol Cryst Commun69: 456-9