Paper Report for: Wu_2022_Comput.Math.Methods.Med_2022_7083022
Reference
Title: TPPU Downregulates Oxidative Stress Damage and Induces BDNF Expression in PC-12 Cells Wu Q, Lin M, Wu P, Zhao C, Yang S, Yu H, Xian W, Song J Ref: Comput Math Methods Med, 2022:7083022, 2022 : PubMed
OBJECTIVE: Ischemia-reperfusion is an ongoing clinical challenge that can lead to a series of pathological changes including oxidative stress. The inhibition of soluble epoxide hydrolase inhibitor (sEH) by 1-(1-propanoylpiperidin-4-yl)-3-[4-(trifluoromethoxy)phenyl]urea (TPPU) results in an anti-inflammatory, cardioprotective, and blood vessel growth-promoting effects. Therefore, this study focused on the protective effect of TPPU on a rat pheochromocytoma (PC-12) cell oxidative stress model induced by H(2)O(2). METHODS: CCK-8 and Hoechst 33342 were used to evaluate cell apoptosis and western blot to detect the apoptotic proteins and brain-derived neurotrophic factor (BDNF) expression. RESULT: The incubation with 100 microM, 50 microM, and 25 microM TPPU significantly increased PC-12 cell viability. Epoxyeicosatrienoic acid (EET) pretreatment also protected PC-12 cells from oxidative stress. In addition, TPPU reduced caspase-3 and Bax expression and induced Bcl-2 expression, and EETs exerted the same effect on caspase-3 expression as TPPU. A positive relationship was found between TPPU or EET incubation and BDNF expression. CONCLUSION: These results revealed that TPPU reduced PC-12 cell oxidative stress injury induced by H(2)O(2) and promoted BDNF expression.
        
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Wu Q, Lin M, Wu P, Zhao C, Yang S, Yu H, Xian W, Song J (2022) TPPU Downregulates Oxidative Stress Damage and Induces BDNF Expression in PC-12 Cells Comput Math Methods Med2022: 7083022
Wu Q, Lin M, Wu P, Zhao C, Yang S, Yu H, Xian W, Song J (2022) Comput Math Methods Med2022: 7083022