Paper Report for: Zhang_2016_Methods.Mol.Biol_1481_29
Reference
Title: Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum Zhang X, He X Ref: Methods Mol Biol, 1481:29, 2016 : PubMed
Wnt proteins are modified and inactivated by two extracellular enzymatic antagonists, Tiki and Notum. Tiki proteins act as membrane-tethered metalloproteases to cleave a fragment from the amino terminus of Wnt proteins. Notum is a Wnt deacylase that removes the lipid modification that is essential for Wnt activities. Here, we provide detailed procedures for preparing enzymatic active Tiki and Notum proteins and the in vitro enzymatic reactions. We also describe a metabolic labeling and click chemistry method for detection of Wnt protein acylation.
        
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Zhang X, He X (2016) Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum Methods Mol Biol1481: 29-38