Medium-ring lactones are synthetically challenging due to unfavorable energetics involved in cyclization. We have discovered a thioesterase enzyme DcsB, from the decarestrictine C1 (1) biosynthetic pathway, that efficiently performs medium-ring lactonizations. DcsB shows broad substrate promiscuity toward linear substrates that vary in lengths and substituents, and is a potential biocatalyst for lactonization. X-ray crystal structure and computational analyses provide insights into the molecular basis of catalysis.
        
Representative scheme of Thiohydrolase structure and an image from PDBsum server
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Databases
PDB-Sum
7D79 Previously Class, Architecture, Topology and Homologous superfamily - PDB-Sum server
FSSP
7D79Fold classification based on Structure-Structure alignment of Proteins - FSSP server