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Substrate Report for: Amplex-red

Amplex Red is a fluorescent probe that is widely used to detect hydrogen peroxide (H2O2) in a reaction where it is oxidised to resorufin by horseradish peroxidase (HRP) as a catalyst. Amplex Red is converted to resorufin by carboxylesterases 1 and 2 without requiring H2O2, horseradish peroxidase or oxygen. Caution is necessary in experimental procedures for the detection of H2O2 release from isolated mitochondria in tissue containing carboxylesterase. It probably is less important for large scale screening of cholinesterases inhibitors


General
Type Phenoxazin, Derivative of Resorufin, Not A/B H target, Fluorescent Probe
Chemical_Nomenclature 1-(3,7-dihydroxyphenoxazin-10-yl)ethanone
Canonical SMILES CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O
InChI InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
InChIKey PKYCWFICOKSIHZ-UHFFFAOYSA-N
Other name(s) 10-Acetyl-3,7-dihydroxyphenoxazine ; 1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone ; ADHP ; Amplex red
________________________________________________________________________________________________
MW|257.24
Formula|C14H11NO4
CAS_number|119171-73-2
PubChem|167453
UniChem|PKYCWFICOKSIHZ-UHFFFAOYSA-N
IUPHAR|
Wikipedia|

Target
Families | Amplex-red ligand of proteins in family: Carb_B_Chordata

References:
Search PubMed for references concerning: Amplex-red

1 more
    Title: Identification of acetylcholinesterase inhibitors using homogenous cell-based assays in quantitative high-throughput screening platforms
    Li S, Huang R, Solomon S, Liu Y, Zhao B, Santillo MF, Xia M
    Ref: Biotechnol J, 12:, 2017 : PubMed

            

    Title: Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays
    Miwa S, Treumann A, Bell A, Vistoli G, Nelson G, Hay S, von Zglinicki T
    Ref: Free Radic Biol Med, 90:173, 2016 : PubMed

            

    Title: A fluorescence assay for measuring acetylcholinesterase activity in rat blood and a human neuroblastoma cell line (SH-SY5Y)
    Santillo MF, Liu Y
    Ref: Journal of Pharmacological & Toxicological Methodsods, 76:15, 2015 : PubMed