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Substrate Report for: N-Benzyl-2-chloroacetamide

Substrate used to test amidase activity of esterases and lipase. corresponding amine released is detected by the formation of a fluorescent dye by coupling with 4-nitro-7-chloro-benzo-2-oxa-1,3-diazole (NBD-CI)


General
Type Amidase substrate
Chemical_Nomenclature N-benzyl-2-chloroacetamide
Canonical SMILES C1=CC=C(C=C1)CNC(=O)CCl
InChI InChI=1S/C9H10ClNO/c10-6-9(12)11-7-8-4-2-1-3-5-8/h1-5H,6-7H2,(H,11,12)
InChIKey SRAXAXHQMCQHSH-UHFFFAOYSA-N
Other name(s) N-Benzyl-2-chloroacetamide ; N-Benzylchloroacetamide ; 2-Chloro-N-benzylacetamide ; Acetamide, 2-chloro-N-(phenylmethyl)-
________________________________________________________________________________________________
MW|183.63
Formula|C9H10ClNO
CAS_number|2564-06-9
PubChem|96233
UniChem|SRAXAXHQMCQHSH-UHFFFAOYSA-N
IUPHAR|
Wikipedia|

Target
Families | N-Benzyl-2-chloroacetamide ligand of proteins in family: Canar_LipB
Protein | canar-LipB

References:
Search PubMed for references concerning: N-Benzyl-2-chloroacetamide
    Title: Bioinformatic analysis of alpha/beta-hydrolase fold enzymes reveals subfamily-specific positions responsible for discrimination of amidase and lipase activities
    Suplatov DA, Besenmatter W, Svedas VK, Svendsen A
    Ref: Protein Engineering Des Sel, 25:689, 2012 : PubMed