Substrate

Bibliography

Biblio print

Tree Display

AceDB Schema

XML Display

Feedback

Substrate Report for: N-(3-Carboxy-4-nitrophenyl)phthalamidic-acid

Chromogenic substrate to analyze . o-phthalyl amidase. Phthalimido is a stable protecting group in beta-lactam chemistry but no conventional chemical methods effectively removes this group. xanag-pham catalyzes the removal of the phthalyl group from phthalyl amides generating phthalate and an amine. The enzyme has a broad substrate specificity and hydrolyzes phthalylated amino acids, peptides, beta-lactams, aromatic and aliphatic amines


General
Type pNP, Phthalate
Chemical_Nomenclature 5-[(2-carboxybenzoyl)amino]-2-nitrobenzoic acid
Canonical SMILES C1=CC=C(C(=C1)C(=O)NC2=CC(=C(C=C2)[N+](=O)[O-])C(=O)O)C(=O)O
InChI InChI=1S/C15H10N2O7/c18-13(9-3-1-2-4-10(9)14(19)20)16-8-5-6-12(17(23)24)11(7-8)15(21)22/h1-7H,(H,16,18)(H,19,20)(H,21,22)
InChIKey ZQXCSGXRVQMUMI-UHFFFAOYSA-N
Other name(s) N-(3-Carboxy-4-nitrophenyl)phthalamidic acid
________________________________________________________________________________________________
MW|330.25
Formula|C15H10N2O7
CAS_number|
PubChem|102001082
UniChem|ZQXCSGXRVQMUMI-UHFFFAOYSA-N
IUPHAR|
Wikipedia|

Target
Families | N-(3-Carboxy-4-nitrophenyl)phthalamidic-acid ligand of proteins in family: 6_AlphaBeta_hydrolase
Protein | xanag-pham

References:
Search PubMed for references concerning: N-(3-Carboxy-4-nitrophenyl)phthalamidic-acid
    Title: o-Phthalyl amidase in the synthesis of loracarbef: Process development using this novel biocatalyst
    Black TD, Briggs BS, Evans R, Muth WL, Vangala S, Zmijewski M
    Ref: Biotechnol Lett, 18:875,  : PubMed

            

    Title: Discovery, purification, and properties of o-phthalyl amidase from Xanthobacter agilis
    Briggs BS, Kreuzman AJ, Whitesitt C, Yeh WK, Zmijewski M
    Ref: J Mol Catal B Enzym, 2:53, 1996 : PubMed