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Substrate Report for: Paranitrophenylacetate

Substrate used in assays for esterase and lipase activity. The release of p-nitrophenol is determined at 405 nm. The molar extinction coefficient for p-nitrophenol is 18,000 M-1 cm-1


General
Type pNP, Chromogen, Aryl ester, Acetate
Chemical_Nomenclature (4-nitrophenyl) acetate
Canonical SMILES CC(=O)OC1=CC=C(C=C1)[N+](=O)[O-]
InChI InChI=1S/C8H7NO4/c1-6(10)13-8-4-2-7(3-5-8)9(11)12/h2-5H,1H3
InChIKey QAUUDNIGJSLPSX-UHFFFAOYSA-N
Other name(s) pNPA ; p-NPA ; pNPA2 ; 4-NPA ; pNP-C2 ; pNP2 ; 4-Nitrophenyl acetate ; P-Acetoxynitrobenzene ; P-Nitrophenol acetate ; p-nitrophenyl acetate ; p-nitrophenyl-acetate ; pNP-acetate ; pNP acetate ; p-NP acetate ; Acetic acid, 4-nitrophenyl ester
________________________________________________________________________________________________
MW|181.15
Formula|C8H7NO4
CAS_number|830-03-5
PubChem|13243
UniChem|QAUUDNIGJSLPSX-UHFFFAOYSA-N
IUPHAR|
Wikipedia|


References:
Search PubMed for references concerning: Paranitrophenylacetate

15 more
    Title: An extended loop in CE7 carbohydrate esterase family is dispensable for oligomerization but required for activity and thermostability
    Singh MK, Manoj N
    Ref: J Struct Biol, 194:434, 2016 : PubMed

            

    Title: Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search
    Porter JL, Boon PL, Murray TP, Huber T, Collyer CA, Ollis DL
    Ref: ACS Chemical Biology, 10:611, 2015 : PubMed

            

    Title: Purification and partial characterization of a novel thermophilic carboxylesterase with high mesophilic specific activity
    Wood AN, Fernandez-Lafuente R, Cowan DA
    Ref: Enzyme Microb Technol, 17:816, 1995 : PubMed

            


eleel-ACHE
MutationKmKcatConditionPaper
WT 5000 uM 76800 /min   Rosenberry_1975_Proc.Natl.Acad.Sci.U.S.A_72_3834
WT 4900 uM 78000 /min   Salih_1992_Biochem.J_285_451

human-ACHE
MutationKmKcatConditionPaper
F338A 8600 uM 20000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
W286A 4600 uM 130000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
W86A 3000 uM 190000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 5600 uM 110000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
Y337A 5400 uM 96000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083

WT Kinetics
Kinetic parametersKmKcatConditionsPapers
eleel-ACHE5000 uM76800 /min  Rosenberry_1975_Proc.Natl.Acad.Sci.U.S.A_72_3834
eleel-ACHE4900 uM78000 /min  Salih_1992_Biochem.J_285_451
human-ACHE5600 uM110000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083