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Torpedo_number Report for: 290

9cucu-ACHE1
MutationKin.TypeEvaluation systemEffectReference
F358S natOP-resistanceWang_2017_Bull.Entomol.Res__1

9neop-ACHE1
MutationKin.TypeEvaluation systemEffectReference
F402V natOP-resistance carbofuran resistanceChang_2014_Pest.Manag.Sci_70_1930

brafl-ACHE2
MutationKin.TypeEvaluation systemEffectReference
F312I sdmAcyl specificityIncrease in kcat for BsChPezzementi_2003_Comp.Biochem.Physiol.B.Biochem.Mol.Biol_136_813
C310A/
F312I		 sdmInactivation by sulfhydrylPezzementi_2006_Invert.Neurosci_6_47

calro-ACHE1A
MutationKin.TypeEvaluation systemEffectReference
F318V natOP-resistanceAgusti-Ridaura_2018_Parasit.Vectors_11_570

colho-ACHE
MutationKin.TypeEvaluation systemEffectReference
F466Y natOP-resistanceda Silva_2011_Vet.Parasitol_177_190

culpi-ACHE1
MutationKin.TypeEvaluation systemEffectReference
F290V natOP-resistance this mutation F416V (290) is found in all resistant mosquitoes from CyprusAlout_2007_Insect.Biochem.Mol.Biol_37_41

cydpo-ACHE1
MutationKin.TypeEvaluation systemEffectReference
F399V natOP-resistancestrain Raz is azinphos-methyl/carbaryl insecticide-resistantCassanelli_2006_Insect.Biochem.Mol.Biol_36_642

drome-ACHE
MutationKin.TypeEvaluation systemEffectReference
F368A nat, sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368A nat, sdmOP-resistanceFournier_1992_J.Biol.Chem_267_14270
F368A nat, sdmOP-resistanceMorton_1982_Biochem.Genet_20_179
F368Y sdm, nat, RandomInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368Y sdm, nat, RandomOP-resistancehigh resistance when combined with other mutationsMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_5922
F368Y sdm, nat, RandomNatural mutantMH19 strain and with other mutations in Saltillo strainMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_5922
F368W sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368W sdmOP biosensorsOP biosensorsBachmann_2000_Biosens.Bioelectron_15_193
F368V sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368S sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368L sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368L sdmBinding of Triton X-100 studiesno effect on Triton X-100 bindingMarcel_2000_J.Biol.Chem_275_11603
F368I sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368H sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368H sdmOP biosensorsOP biosensorsBachmann_2000_Biosens.Bioelectron_15_193
F368G sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F368C sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
F115S/
F368Y		 sdmOP-resistanceOP-resistance lower than multiple,mut found in Saltillo strain together with I199V G303A F368YMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_5922
F115S/
I199V/
F368Y		 sdmOP-resistanceOP-resistance lower than multiple,mut found in Saltillo strain together with I199V G303A F368YMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_5922
F115S/
G303A/
F368Y		 sdmOP-resistanceOP-resistance lower than multiple,mut found in Saltillo strain together with I199V G303A F368YMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_592
F115S/
I199V/
G303A/
F368Y		 natNatural mutationSaltillo strain OP-resistanceMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_5922
I199V/
F368Y		 sdmOP-resistanceTwo mutations found in Saltillo strainMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_5922
I199V/
G303A/
F368Y		 sdmOP-resistanceThree mutations found in OP-resistant Saltillo strainMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_5922
G303A/
F368Y		 sdmOP-resistanceFound associated F115S and I199V mutations in Saltillo strain, high resistance for propoxure malaoxon and carbarylMutero_1994_Proc.Natl.Acad.Sci.U.S.A_91_592

human-ACHE : (kinetic parameters)
MutationKin.TypeEvaluation systemEffectReference
F297I sdmOxime interactionCochran_2011_J.Biol.Chem_286_29718
F297I/
Y337A		 sdmOxime interactionCochran_2011_J.Biol.Chem_286_29718
E202Q/
F297I/
Y337A		 sdmOxime interactionCochran_2011_J.Biol.Chem_286_29718
F297VkinsdmAcyl specificity Acyl pocket, increases Km for ATC and increase kcatOrdentlich_1993_J.Biol.Chem_268_17083,
Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097
F297VkinsdmAcyl specificityreplacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHEKaplan_2001_Biochemistry_40_7433
F295L/
F297V		kinsdmAcyl specificityAcyl pocket, Increases hydrolysis of BTCOrdentlich_1993_J.Biol.Chem_268_17083,
Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097,
Ashani_1994_Mol.Pharmacol_45_555
F295L/
F297V		kinsdmOP-specificityincreases reactivity towards DFP DEFP and paraoxonOrdentlich_1996_J.Biol.Chem_271_11953'
F295L/
F297V		kinsdmOP-specificitydecrease in stereoselectivityOrdentlich_2004_Biochemistry_43_11255
F295L/
F297V		kinsdmAcyl specificityreplacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHEKaplan_2001_Biochemistry_40_7433
F295L/
F297V/
Y337A		kinsdmAcyl specificityreplacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHEKaplan_2001_Biochemistry_40_7433,
Barak_2005_Chem.Biol.Interact_157-158_219
Y72N/
Y124Q/
W286A/
F295L/
F297W/
Y337A		 sdmAcyl specificityreplacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHEKaplan_2001_Biochemistry_40_7433
Y72N/
Y124Q/
W286A/
F295L/
F297V/
Y337A/
V407F		 sdmAcyl specificity Acyl pocket V407F does not increase kcat of the hexamutantBarak_2002_Biochemistry_41_8245
F295A/
F297A		 sdmAcyl specificityVX stereospecificity decrease in stereoselectivityOrdentlich_2005_Chem.Biol.Interact_157-158_191
F297AkinsdmAcyl specificity Acyl pocket, increases Km for ATC and increase kcatOrdentlich_1993_J.Biol.Chem_268_17083,
Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097,
Barak_1994_J.Biol.Chem_269_6296,
Ordentlich_1995_5th.ChE.Meeting.Madras__221
Y72N/
Y124Q/
W286A/
F295L/
F297V/
Y337A		kinsdmAcyl specificityreplacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHEKaplan_2001_Biochemistry_40_7433

human-BCHE
MutationKin.TypeEvaluation systemEffectReference
T284P/
S287R/
V288S		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
T284Q/
P285S/
L286I/
S287H/
V288I		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
T284P/
P285S/
L286H/
V288G		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
T284N/
P285H/
L286I/
V288G		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
T284H/
P285T/
L286I/
S287H/
V288G		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
V288H sdmSearch for phosphotriesterase activityPhosphotriesterase activity Identical to WTLockridge_1997_Biochemistry_36_786

luccu-ACHE
MutationKin.TypeEvaluation systemEffectReference
F368Y sdmInsecticide sensitivityChen_2001_Insect.Biochem.Mol.Biol_31_805
F115S/
I199V/
F368Y		 sdmOP-resistanceChen_2001_Insect.Biochem.Mol.Biol_31_805
I199V/
F368Y		 sdmOP-resistanceChen_2001_Insect.Biochem.Mol.Biol_31_805
I199V/
G303A/
F368Y		 sdmOP-resistanceChen_2001_Insect.Biochem.Mol.Biol_31_805
G303A/
F368Y		 sdmOP-resistanceChen_2001_Insect.Biochem.Mol.Biol_31_805

luccu-E3aest7
MutationKin.TypeEvaluation systemEffectReference
F309L sdmOP-resistancesearch for OP hydrolase and MCE activities Heidari_2004_Insect.Biochem.Mol.Biol_34_353
F309L sdmPyrethroids hydrolysissearch for Pyrethroids hydrolysis activities Heidari_2005_Insect.Biochem.Mol.Biol_35_597
F309L sdmPyrethroids hydrolysisHydrolysis of individual isomers of fluorogenic pyrethroid analogs by mutant carboxylesterasesDevonshire_2007_Insect.Biochem.Mol.Biol_37_891
W251L/
F309L		 sdmOP-resistancesearch for OP hydrolase and MCE activities Heidari_2004_Insect.Biochem.Mol.Biol_34_353
W251L/
F309L		 sdmPyrethroids hydrolysissearch for Pyrethroids hydrolysis activities Heidari_2005_Insect.Biochem.Mol.Biol_35_597
W251L/
F309L		 sdmPyrethroids hydrolysisHydrolysis of individual isomers of fluorogenic pyrethroid analogs by mutant carboxylesterasesDevonshire_2007_Insect.Biochem.Mol.Biol_37_891

mouse-ACHE : (kinetic parameters)
MutationKin.TypeEvaluation systemEffectReference
F297AkinsdmAcyl specificityAcyl pocket specificity of chiral inhibitorsHosea_1995_Biochemistry_34_11528
F295L/
F297I		kinsdmAcyl specificityAcyl pocket, reduced catalytic activity for ACT and enhanced for BCTVellom_1993_Biochemistry_32_12
F295L/
R296S/
F297I		 sdmAcyl specificityAcyl pocket reduced catalytic activity for ACT and enhanced for BCTVellom_1993_Biochemistry_32_12
F297IkinsdmAcyl specificityAcyl pocket, increases Km for ATC and increase kcatVellom_1993_Biochemistry_32_12,
Pickering_1995_5th.ChE.Meeting.Madras__227
F297IkinsdmSubstrate inhibitionthis BChE residue mutation eliminates substrate inhibition partially mimics this activation behaviorVellom_1993_Biochemistry_32_12,
Radic_1993_Biochemistry_32_12074
F297IkinsdmFasciculin inhibition low effect on KiRadic_1994_J.Biol.Chem_269_11233
F297IkinsdmAricept~Donepezil~E2020 inhibitionincrease Ki vs WTSaxena_2003_Eur.J.Biochem_270_4447
F297IkinsdmOxime interactionenlargement of the active site of the gorgeKovarik_2006_Biochem.Biophys.Res.Commun_342_973,
Kovarik_2007_Toxicology_233_79
F297YkinsdmAcyl specificityAcyl pocket, increase km decrease kcat ATCRadic_1993_Biochemistry_32_12074,
Radic_1994_J.Biol.Chem_269_11233,
Pickering_1995_5th.ChE.Meeting.Madras__227
F297YkinsdmSubstrate inhibitionhighRadic_1993_Biochemistry_32_12074,
Radic_1994_J.Biol.Chem_269_11233,
Pickering_1995_5th.ChE.Meeting.Madras__227
F297I/
Y337A		 sdmOxime interactionenlargement of the active site of the gorgeKovarik_2006_Biochem.Biophys.Res.Commun_342_973,
Kovarik_2007_Toxicology_233_79
F297I/
Y337A		 sdmOxime interactionKovarik_2004_Biochemistry_43_3222
F295L/
F297I/
Y337A		 sdmoxime interactionenlargement of the active site of the gorgeKovarik_2006_Biochem.Biophys.Res.Commun_342_973
F295L/
F297I/
Y337A		 sdmOxime interactionKovarik_2004_Biochemistry_43_3222

musdo-ACHE
MutationKin.TypeEvaluation systemEffectReference
V180L/
G262A/
F327Y		 natOP-resistancemutations found in the 77M strain resistant to methomylWalsh_2001_Biochem.J_359_175
V180L/
G262A/
F327Y		 natOP-resistancemutations found in the SH-CBR strain resistant to carbamateLiming_2006_Pestic.Biochem.Physiol_86_1
V180L/
G262A/
F327Y		 natOP-resistanceBaskurt_2011_J.Vector.Ecol_36_248
V180L/
G262A/
F327Y/
D341L		 natOP-resistancemutations found in the SH-CBR strain resistant to carbamateLiming_2006_Pestic.Biochem.Physiol_86_1
V180L/
G262A/
F327Y/
D341L		 natOP-resistanceDanish housefly strain 571ab from tokyo fenitrothionKristensen_2006_Pest.Manag.Sci_62_738
G262A/
F327Y		 natOP-resistancemutations found in the CH2 strain resistant to methomylWalsh_2001_Biochem.J_359_175
G262A/
F327Y		 natOP-resistanceDanish housefly strain 690ab from Denmark methomylKristensen_2006_Pest.Manag.Sci_62_738
G262A/
F327Y		 natOP-resistanceBaskurt_2011_J.Vector.Ecol_36_248
G262V/
F327Y		 natOP-resistancemutations found in the 690ab strain resistant to methomylWalsh_2001_Biochem.J_359_175
G262V/
F327Y		 natOP-resistanceDanish housefly strain 690ab Denmark methomylKristensen_2006_Pest.Manag.Sci_62_738
G262V/
F327Y		 natOP-resistanceBaskurt_2011_J.Vector.Ecol_36_248
T230M/
G262V/
F327Y/
A586T		 natOP-resistanceDanish housefly strain 594vb Denmark azamethiphos 381zb Denmark multi resistant OP carbamate pyrthroidKristensen_2006_Pest.Manag.Sci_62_738
F327Y natOP-resistanceinsensitivity to fenitroxonKozaki_2001_Insect.Biochem.Mol.Biol_31_991
I82M/
G262A/
F327Y		 natOP-resistancerestoring G262 and F327 restore sensitivity Kim_2003_Mol.Cells_15_208

nipbr-ACHEB
MutationKin.TypeEvaluation systemEffectReference
M301A/
W303A		 sdmInsecticide sensitivitySchulze_2005_Anal.Chem_77_5823
M301A/
W303L		 sdmInsecticide sensitivitySchulze_2005_Anal.Chem_77_5823
W303A sdmInsecticide sensitivitySchulze_2005_Anal.Chem_77_5823
W303A/
26aaIns		 sdmInsecticide sensitivityVillatte_2003_Protein.Eng_16_463
W303G sdmInsecticide sensitivitySchulze_2005_Anal.Chem_77_5823
W303L sdmInsecticide sensitivitySchulze_2005_Anal.Chem_77_5823

pluxy-ACHE1
MutationKin.TypeEvaluation systemEffectReference
F386V natOP-resistanceResistance to Mevinphos G227A A201S, F386V, (G324A,A298S, F386F/V)Yeh_2014_Pestic.Biochem.Physiol_112_7

rhopd-ACHE2
MutationKin.TypeEvaluation systemEffectReference
F368L natOP-resistanceChen_2007_Genome_50_172

spolt-ACHE1
MutationKin.TypeEvaluation systemEffectReference

torma-ACHE
MutationKin.TypeEvaluation systemEffectReference
F288L/
F290V		 sdmAcyl specificity Acyl pocket hydrolysis of BTC as well as ATC , inhibited also by iso-OMPAHarel_1992_Proc.Natl.Acad.Sci.U.S.A_89_10827
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