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Torpedo_number Report for: 443
human-ACHE
:
(
kinetic parameters
)
Mutation
Kin.
Type
Evaluation system
Effect
Reference
E450A
kin
sdm
Catalysis
Catalysis/H-bond network/active center modulation, reduced kapp for ACT and TB
Ordentlich_1993_FEBS.Lett_334_215
,
Ordentlich_1995_5th.ChE.Meeting.Madras__221
,
Shafferman_1995_5th.ChE.Meeting.Madras__189
E450A
kin
sdm
Heat and pressure stability
strengthened heat stability decreased pressure stability
Clery-Barraud_2002_Eur.J.Biochem_269_4297
human-BCHE
Mutation
Kin.
Type
Evaluation system
Effect
Reference
A199S/
F227A/
S287G/
A328W/
E441D
sdm
Cocaine hydrolysis
improved catalytic efficiency against-cocaine E
Xue_2011_Mol.Pharmacol_79_290
D70G/
E441G/
E443Q
sdm
Succinylcholine hydrolysis
Catalysis reduced activity/Succinylcholine hydrolysis/Dibucaine inhibition 441 and 443 bind succinylcholine but mutant do not bind dibucaine
Neville_1992_EMBO.J_11_1641
D70G/
E441G/
E443Q
sdm
Catalysis
Reduced activity
Neville_1992_EMBO.J_11_1641
E441G/
E443Q
sdm
Catalysis
drastically reduced activity towards BTC modifies the eletronegativ envir of H438
Neville_1992_EMBO.J_11_1641
,
Soreq_1992_Trends.Biochem.Sci_17_353
E441G/
E443Q
sdm
Succinylcholine hydrolysis
drastically reduced activity towards BTC modifies the eletronegativ envir of H438
Neville_1992_EMBO.J_11_1641
,
Soreq_1992_Trends.Biochem.Sci_17_353
E441G/
E443Q
sdm
Dibucaine inhibition
resistant to dibucaine
Neville_1992_EMBO.J_11_1641
,
Soreq_1992_Trends.Biochem.Sci_17_353
mouse-ACHE
Mutation
Kin.
Type
Evaluation system
Effect
Reference
E450Q
sdm
Electrostatic
influence TFK inhibition but not ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
D74N/
E202Q/
E450Q
sdm
Electrostatic
influence TFK inhibition but not ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
Send your questions or comments to :
Mail to:
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite:
Lenfant 2013 Nucleic.Acids.Res.
or
Marchot Chatonnet 2012 Prot.Pept Lett.
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