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Torpedo_number Report for: 82
human-ACHE
:
(
kinetic parameters
)
Mutation
Kin.
Type
Evaluation system
Effect
Reference
E84Q
kin
sdm
Omega loop
No effect
Velan_1996_FEBS.Lett_395_22
E84Q
kin
sdm
Electrostatic
Electrostatic carboxyl/attraction studies Little effect on hydrolysis or inhibition
Shafferman_1992_J.Biol.Chem_267_17640
,
Shafferman_1992_4th.ChE.Meeting.Eilat__165
,
Kronman_1994_J.Biol.Chem_269_27819
,
Shafferman_1994_EMBO.J_13_3448
E84Q/
E292A
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
D349N/
E358Q
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
E292A/
D349N/
E358Q
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
E285A/
E292A/
D349N/
E358Q
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
D349N/
E358Q/
E389Q/
D390N
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
E292A/
D349N/
E358Q/
E389Q/
D390N
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
E292A/
D349N/
E358Q/
E389Q/
D390N
kin
sdm
Heat and pressure stability
strengthened heat stability decreased pressure stability
Clery-Barraud_2002_Eur.J.Biochem_269_4297
E84Q/
E285A/
E292A/
D349N/
E358Q/
E389Q/
D390N
kin
sdm
Electrostatic
Electrostatic attraction hypothesisno significant effect
Shafferman_1994_EMBO.J_13_3448
mouse-ACHE
Mutation
Kin.
Type
Evaluation system
Effect
Reference
E84C
sdm
Omega loop
Omega loop conformation test incorporation of the fluorophore acrylodan
Shi_2001_J.Biol.Chem_276_42196
E84Q
sdm
Electrostatic
electrostatic influence reduced, influences FAS2 inhibition and its ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
E84Q/
E91Q
sdm
Electrostatic
electrostatic influence reduced, influences FAS2 inhibition and its ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
E84Q/
E91Q/
D280V/
D283N
sdm
Electrostatic
electrostatic influence reduced, influences FAS2 inhibition and its ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
E84Q/
E91Q/
D280V/
D283N/
D372N
sdm
Electrostatic
electrostatic influence reduced, influences FAS2 inhibition and its ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
E84Q/
E91Q/
D280V/
D283N/
E292Q/
D372N
sdm
Electrostatic
electrostatic influence reduced, influences FAS2 inhibition and its ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
Send your questions or comments to :
Mail to:
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite:
Lenfant 2013 Nucleic.Acids.Res.
or
Marchot Chatonnet 2012 Prot.Pept Lett.
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