Mutation Report for: A199S/F227A/S287G/A328W/Y332G/F364C/M532C_human-BCHE

A199S/F227A/S287G/A328W/Y332G/F364C/M532C_human-BCHE	Gene_locus	human-BCHE
	Torpedo_number	201
		229
		289
		330
		334
		366
		534
		201,229,289,330,334,365,534
	AA_Change	A199S/F227A/S287G/A328W/Y332G/F364C/M532C
	Mode_of_mutation	Site directed mutagenesis
	Modification	Cocaine hydrolysis
	Summary	Cocaine hydrolysis;improved catalytic efficiency against-cocaine and increased biological half-life with new cross-subunit SS bonds;Fang_2014_Chem.Biol.Interact_214C_18
	Paper	Fang_2014_Chem.Biol.Interact_214C_18
	Comment	p.A199S/F227A/S287G/A328W/Y332G/F364C/M532C Ala199Ser/Phe227Ala/Ser287Gly/Ala328Trp/Tyr332Gly/Phe364Cys/M532Cys (p.A227S/F255A/S315G/A356W/Y360G/F392C/M560C Ala227Ser/Phe255Ala/Ser315Gly/Ala356Trp/Tyr360Gly/Phe392Cys/Met560Cys in primary sequence with 28 amino-acids signal peptide) denoted E364-532 for convenience. Mutations F364C/M532C introduce a new disulfide cross-subunit bond on the BChE mutated for increased cocaine hydrolysis

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

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