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Mutation Report for: A199S/F227A/S287G/A328W/Y332G/F364C/M532C_human-BCHE

Name Class
A199S/F227A/S287G/A328W/Y332G/F364C/M532C_human-BCHEGene_locushuman-BCHE
Torpedo_number (8)
AA_ChangeA199S/F227A/S287G/A328W/Y332G/F364C/M532C
Mode_of_mutationSite directed mutagenesis
ModificationCocaine hydrolysis
SummaryCocaine hydrolysis;improved catalytic efficiency against-cocaine and increased biological half-life with new cross-subunit SS bonds;Fang_2014_Chem.Biol.Interact_214C_18
PaperFang_2014_Chem.Biol.Interact_214C_18
Commentp.A199S/F227A/S287G/A328W/Y332G/F364C/M532C Ala199Ser/Phe227Ala/Ser287Gly/Ala328Trp/Tyr332Gly/Phe364Cys/M532Cys (p.A227S/F255A/S315G/A356W/Y360G/F392C/M560C Ala227Ser/Phe255Ala/Ser315Gly/Ala356Trp/Tyr360Gly/Phe392Cys/Met560Cys in primary sequence with 28 amino-acids signal peptide) denoted E364-532 for convenience. Mutations F364C/M532C introduce a new disulfide cross-subunit bond on the BChE mutated for increased cocaine hydrolysis

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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