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Mutation Report for: E387D_human-BCHE

Name Class
E387D_human-BCHEGene_locushuman-BCHE
Torpedo_number389
AA_ChangeE387D
Mode_of_mutationSite directed mutagenesis
ModificationExpression in E Coli
PROSS algorithm
SummaryPROSS algorithm;Expression in E Coli;Brazzolotto_2017_Molecules_22_
PaperBrazzolotto_2017_Molecules_22_
Structure6EMI
Commentp.E387D Glu387Asp (p.E415D Glu415Asp in primary sequence with 28 amino-acids signal peptide) Mutation designed by -Protein Repair One Stop Shop- process for the production of active human butyrylcholinesterase in a prokaryotic system. The mutation gives Asp as in ACHE of Torpedo californica and mouse

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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