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Mutation Report for: E84Q/E292A/D349N/E358Q/E389Q/D390N_human-ACHE

Name Class
E84Q/E292A/D349N/E358Q/E389Q/D390N_human-ACHEGene_locushuman-ACHE
Torpedo_number (7)
AA_ChangeE84Q/E292A/D349N/E358Q/E389Q/D390N
Mode_of_mutationSite directed mutagenesis
ModificationElectrostatic
Heat and pressure stability
Stability
SummaryElectrostatic;Electrostatic attraction hypothesis no significant effect;Shafferman_1994_EMBO.J_13_3448
Heat and pressure stability;strengthened heat stability decreased pressure stability;Clery-Barraud_2002_Eur.J.Biochem_269_4297
PaperShafferman_1994_EMBO.J_13_3448
Clery-Barraud_2002_Eur.J.Biochem_269_4297
Kinetic_parameterAcetylthiocholine_E84Q/E292A/D349N/E358Q/E389Q/D390N_human-ACHE
3,3-dimethylbutylthioacetate_E84Q/E292A/D349N/E358Q/E389Q/D390N_human-ACHE
Edrophonium_E84Q/E292A/D349N/E358Q/E389Q/D390N_human-ACHE
Commentp.E84Q/E292A/D349N/E358Q/E389Q/D390N Glu84Gln/Glu292Ala/Asp349Asn/Glu358Gln/Glu389Gln/Asp390Asn (p.E115Q/E323A/D380N/E389Q/E420Q/D421N Glu115Gln/Glu323Ala/Asp380Asn/Glu389Gln/Glu420Gln/Asp421Asn in primary sequence with 31 amino-acids signal peptide) Electrostatic attraction hypothesis no significant effect
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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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