p.L274D Leu274Asp (p.L302D Leu302Asp in primary sequence with 28 amino-acids signal peptide) Mutation designed by -Protein Repair One Stop Shop- process for the production of active human butyrylcholinesterase in a prokaryotic system. The mutation gives Leu as in ACHE of Torpedo Californica and human and mouse Bungarus fasciatus
