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Mutation Report for: L274D_human-BCHE

Name Class
L274D_human-BCHEGene_locushuman-BCHE
Torpedo_number276
AA_ChangeL274D
Mode_of_mutationSite directed mutagenesis
ModificationExpression in E Coli
PROSS algorithm
SummaryPROSS algorithm;Expression in E Coli;Brazzolotto_2017_Molecules_22_
PaperBrazzolotto_2017_Molecules_22_
Structure6EMI
Commentp.L274D Leu274Asp (p.L302D Leu302Asp in primary sequence with 28 amino-acids signal peptide) Mutation designed by -Protein Repair One Stop Shop- process for the production of active human butyrylcholinesterase in a prokaryotic system. The mutation gives Leu as in ACHE of Torpedo Californica and human and mouse Bungarus fasciatus

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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