Mutation Report for: Y341A_human-ACHE

Y341A_human-ACHE	Gene_locus	human-ACHE
	Torpedo_number	334
	AA_Change	Y341A
	Mode_of_mutation	Site directed mutagenesis
	Modification	Peripheral Anionic Site
		Omega loop interaction
		Oxime interaction
	Summary	Omega loop interaction;No effect;Velan_1996_FEBS.Lett_395_22
		Peripheral Anionic Site;low decrease in affinity for edrophonium, no inhib by excess substrate;Shafferman_1992_EMBO.J_11_3561;Shafferman_1992_4th.ChE.Meeting.Eilat__165;Barak_1994_J.Biol.Chem_269_6296;Shafferman_1995_5th.ChE.Meeting.Madras__189
		oxime interaction;reactivation of tabun-inhibited human AChE. The dissociation constant 3.5-fold increased;Artursson_2009_Toxicology_265_108
	Paper (7)
	Kinetic_parameter (8)
	Comment	p.Y341A Tyr341Ala (p.Y372A Tyr372Ala in primary sequence with 31 amino-acids signal peptide) Omega loop interaction;No effect - Peripheral Anionic Site;low decrease in affinity for edrophonium, no inhib by excess substrate -oxime interaction;reactivation of tabun-inhibited human AChE. The dissociation constant 3.5-fold increased

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

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