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Mutation Report for: Y72N/Y124Q/W286A/F295L/F297V/Y337A_human-ACHE

Name Class
Y72N/Y124Q/W286A/F295L/F297V/Y337A_human-ACHEGene_locushuman-ACHE
Torpedo_number70
121
279
288
290
330
70,121,279,288,290,330
AA_ChangeY72N/Y124Q/W286A/F295L/F297V/Y337A
Mode_of_mutationSite directed mutagenesis
ModificationAcyl specificity
SummaryAcyl specificity;replacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHE;Kaplan_2001_Biochemistry_40_7433
PaperKaplan_2001_Biochemistry_40_7433
Kinetic_parameterAcetylthiocholine_Y72N/Y124Q/W286A/F295L/F297V/Y337A_human-ACHE
Butyrylthiocholine_Y72N/Y124Q/W286A/F295L/F297V/Y337A_human-ACHE
CommentY72N/Y124Q/W286A/F295L/F297V/Y337A p.Tyr72Asn/Tyr124Gln/Trp286Ala/Phe295Leu/Phe297Val/Tyr337Ala (Y103N/Y155Q/W317A/F326L/F328V/Y368A p.Tyr103Asn/Tyr124Gln/Trp317Ala/Phe326Leu/Phe328Val/Tyr368Ala in primary sequence with 31 amino-acids signal peptide) Acyl specificity;replacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHE

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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