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Gene_locus Report for: ecoli-T6SS.TLE3

Name Class
ecoli-T6SS.TLE3Name Escherichia coli Adherent-Invasive Tle3 T6SS Effector LF82_435
SpeciesEscherichia coli
Gene_nameNRG857_13830
FPI65_17315
LF82_435
FamilyT6SS-TLE3
DatabaseSwissProtA0A2G9AAY6 A0A2G9AAY6_ECOLX
A0A0H3ENW7 A0A0H3ENW7_ECO8N
A0A0H2VAD3 A0A0H2VAD3_ECOL6
Genbank (6)
TremblA0A2G9AAY6
A0A0H3ENW7
A0A0H2VAD3
E2QQR9
NCBI_TaxID562
685038
199310
PaperLe_2023_Int.J.Mol.Sci_24_1740
Russell_2013_Nature_496_508
CommentFamily of Tle3 phospholipase effector .The type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have been identified as antibacterial effectors that hydrolyze membrane phospholipids. The Tle1-4 display phospholipase A1 and A2 (PLA1) (PLA2) activities. Tle1 belongs to Duf_2235 (T6SS-TLE1) family, TLE2 to Lipase_3, TLE3 to alpha/ beta hydrolase and Tle4 belongs to PGAP1 (Tle5 family, such as Pseudomonas aeruginosa PldA and PldB, are phospholipases D and are not alpha/beta hydrolases). Tle3 phospholipase effector from adherent-invasive Escherichia coli (AIEC) Tle3AIEC possesses phospholipase A1 (PLA1) activity (specific activity (SA) = 7.65 nmole.min-1.mg-1) but no PLA2 activity. . The T6SS-TLE3 is decorated by two protruding segments, including a N-terminus loop containing part of the oxyanion hole residues. The long C-terminus contains the catalytic site histidine and in some members is related to the DUF3274 family (most members of this family are not linked to alpha/beta hydrolases).In T6SS, protection against its own toxic effector is ensured in bacteria by the production of immunity proteins that specifically bind and inhibit Tle3. Tli (Type VI lipase immunity) binds tightly to Tle3. There is also a strong interaction between the VgrGAIEC C-terminus adaptor and Tle3AIEC N-terminal loop. Other strains: Escherichia coli O83:H1 (strain NRG 857C / AIEC); Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Proteinecoli-T6SS.TLE3
Peptideecoli-T6SS.TLE3
Structure8BOZ
SubstratePED-A1
Tree_nodeT6SS-TLE3300153

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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