Type Substrate

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Type_substrate Report for: pNP

Comment

Esters of 4-Nitrophenol (also called p-nitrophenol or 4-hydroxynitrobenzene) which is a phenolic compound that has a nitro group at the opposite position of the hydroxyl group on the benzene ring. Amounts of 4-nitrophenol produced by a particular enzyme in the presence of its corresponding substrate can be measured with a spectrophotometer at or around 405 nm and used as a proxy measurement for the amount of the enzyme activity in the sample.

Substrate nameFormulaStructure
Ac-Leu-p-nitroanilideC14H19N3O4
Ala-Pro-pNAC14H18N4O4
Arg-Pro-pNAC17H25N7O4
BAPNAC19H22N6O4
ChloramphenicolC11H12Cl2N2O5
Chloramphenicol-diacetateC15H16Cl2N2O7
Gly-Phe-pNAC17H18N4O4
Gly-Pro-pNAC13H16N4O4
Me-GlcA-pNPC13H15NO9
MpNPTC14H9NO6
N-(3-Carboxy-4-nitrophenyl)phthalamidic-acidC15H10N2O7
Naproxen-p-nitrophenol-esterC20H19NO5
P-nitrophenyl-phosphateC6H6NO6P
Paranitrobenzylacetate~PNBAC9H9NO4
ParanitrobutyranilideC10H12N2O3
Paranitrophenyl-2-methyldecanoateC17H25NO4
Paranitrophenyl-N-benzylcarbamateC13H10N2O4
Paranitrophenyl-decanoateC16H23NO4
Paranitrophenyl-hexanoateC12H15NO4
Paranitrophenyl-laurateC18H27NO4
Paranitrophenyl-myristateC20H31NO4
Paranitrophenyl-octanoateC14H19NO4
Paranitrophenyl-palmitateC22H35NO4
Paranitrophenyl-stearateC24H39NO4
ParanitrophenylacetateC8H7NO4
ParanitrophenylbutyrateC10H11NO4
ParanitrophenylpropionateC9H9NO4
ParanitrophenylthioacetateC8H7NO3S
ParanitrophenylvalerateC11H13NO4
ParaoxonC10H14NO6P
Z-Gly-Pro-pNAC21H22N4O6
butyl-N(para-nitrophenyl)carbamateC11H14N2O4
pNPXC11H13NO7

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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