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Type_substrate Report for: pNP

Esters of 4-Nitrophenol (also called p-nitrophenol or 4-hydroxynitrobenzene) which is a phenolic compound that has a nitro group at the opposite position of the hydroxyl group on the benzene ring. Amounts of 4-nitrophenol produced by a particular enzyme in the presence of its corresponding substrate can be measured with a spectrophotometer at or around 405 nm and used as a proxy measurement for the amount of the enzyme activity in the sample.
See also this moiety in type of inhibitors: pNP

SubstrateMain targeted enzyme familiesTypeStructure
Ac-Leu-p-nitroanilideACPH_Peptidase_S9 Chromogen | Peptide | p-nitroaniline | pNP
Ala-Pro-Ala-pNaTiancimycin-TnmK-Tripeptidase-HIP Carboxamide | Chromogen | Peptide | Pyrrolidine | p-nitroaniline | pNP
Ala-Pro-pNADPP4N_Peptidase_S9 Carboxamide | Chromogen | Peptide | Pyrrolidine | p-nitroaniline | pNP
Arg-Pro-pNADPP4N_Peptidase_S9 Carboxamide | Chromogen | Peptide | Pyrrolidine | p-nitroaniline | pNP
BAPNAS9N_PREPL_Peptidase_S9 Peptide | p-nitroaniline | pNP
ChloramphenicolHormone-sensitive_lipase_like Antibiotic | pNP
Chloramphenicol-diacetate Acetate | Antibiotic | pNP
DNPGPAF-Acetylhydrolase Choline ester | pNP
Gly-Phe-pNADPP4N_Peptidase_S9 | Prolyl_oligopeptidase_S9 Carboxamide | Chromogen | Peptide | p-nitroaniline | pNP
Gly-Pro-pNADPP4N_Peptidase_S9 | Prolylcarboxypeptidase Carboxamide | Chromogen | Peptide | Pyrrolidine | p-nitroaniline | pNP
Me-GlcA-pNPGlucuronoyl_esterase Carbohydrate | Glucuronoyl | Glycoside | pNP
MpNPTTannase Chromogen | Phthalate | Terephthalate | pNP
N-(3-Carboxy-4-nitrophenyl)phthalamidic-acid6_AlphaBeta_hydrolase Phthalate | pNP
NPh-2-Fe-ArafEsterase_phb Feruloyl | Glycoside | pNP
NPh-4-Fe-XylpEsterase_phb Feruloyl | Glycoside | pNP
NPh-5-Fe-ArafEsterase_phb Feruloyl | Glycoside | pNP
NPh-Xyl-MeGlcAGlucuronoyl_esterase Carbohydrate | Glucuronoyl | Glycoside | pNP
Naproxen-p-nitrophenol-esterHormone-sensitive_lipase_like Chromogen | Naphthalen | Propionate | pNP
P-nitrophenyl-phosphateACPH_Peptidase_S9 Chromogen | Organophosphate | pNP
Paranitrobenzylacetate~PNBACarb_B_Bacteria Acetate | Aryl ester | Chromogen | pNP
ParanitrobutyranilideCanar_LipB | Carb_B_Bacteria | Cutinase Amidase substrate | Chromogen | pNP
Paranitrophenyl-2-methyldecanoate Aryl ester | Chromogen | pNP
Paranitrophenyl-N-benzylcarbamateCutinase Carbamate | Chromogen | Polyurethane | pNP
Paranitrophenyl-decanoateBact_LipEH166_FamXII | Bacterial_lip_FamI.5 | CarbLipBact_2 | Fungal_carboxylesterase_lipase | GTSAGmotif | Lipase_2 | Lipase_bact_N_lipase Chromogen | Decanoate | Water-insoluble medium and long chain ester | pNP
Paranitrophenyl-ferulateFAE-Bacterial-promiscuous | Lipase_3 Acrylate | Chromogen | Feruloyl | Hydroxycinnamic-ester | pNP
Paranitrophenyl-heptanoate Chromogen | Water-insoluble medium and long chain ester | pNP
Paranitrophenyl-hexanoateABHD11-Acetyl_transferase | Bacterial_Est97 | Bacterial_lip_FamI.2 | Est9X | Fungal-Bact_LIP | Fungal_carboxylesterase_lipase | GTSAGmotif | Hormone-sensitive_lipase_like | Lipase_2 | PE-PPE | Polyesterase-lipase-cutinase Chromogen | Water-soluble short chain ester | pNP
Paranitrophenyl-laurateBact_LipEH166_FamXII | Cutinase | Fungal_carboxylesterase_lipase | Hormone-sensitive_lipase_like | LYsophospholipase_carboxylesterase | Lipase_2 | Lipase_bact_N_lipase | Monoglyceridelipase_lysophospholip Chromogen | Dodecanoate | Water-insoluble medium and long chain ester | pNP
Paranitrophenyl-myristateABHD6-Lip | Bact_LipEH166_FamXII | Fungal-Bact_LIP | Fungal_carboxylesterase_lipase | Hormone-sensitive_lipase_like | Lipase_2 | Monoglyceridelipase_lysophospholip Chromogen | Tetradecanoate | Water-insoluble medium and long chain ester | pNP
Paranitrophenyl-octanoate6_AlphaBeta_hydrolase | ABHD6-Lip | ACPH_Peptidase_S9 | AlphaBeta_hydrolase | Bact_LipEH166_FamXII | Bacterial_EstLip_FamX | Bacterial_lip_FamI.5 | Bacterial_lip_FamI.6 | CarbLipBact_2 | Cutinase | Fungal_carboxylesterase_lipase | GTSAGmotif | Hormone-sensitive_lipase_like | LYsophospholipase_carboxylesterase | Lipase_2 | Lipase_3 | Pectinacetylesterase-Notum | Polyesterase-lipase-cutinase Chromogen | Octanoate | Water-insoluble medium and long chain ester | pNP
Paranitrophenyl-palmitateCutinase | Fungal-Bact_LIP | Fungal_carboxylesterase_lipase | Hormone-sensitive_lipase_like | Lipase_2 | Lipase_3 | Monoglyceridelipase_lysophospholip Chromogen | Hexadecanoate | Palmitate | Water-insoluble medium and long chain ester | pNP
Paranitrophenyl-stearateFungal_carboxylesterase_lipase | Lipase_2 Chromogen | Water-insoluble medium and long chain ester | pNP
Paranitrophenylacetate6_AlphaBeta_hydrolase | A85-EsteraseD-FGH | A85-Feruloyl-Esterase | ACHE | Acetyl-esterase_deacetylase | BCHE | Bacterial_esterase | Bacterial_lip_FamI.5 | BioH | CIB-CCG1-interacting-factor-B | CarbLipBact_1 | Carb_B_Bacteria | Carb_B_Chordata | Carboxylesterase | Cutinase | Est9X | FaeC | Fungal_carboxylesterase_lipase | GTSAGmotif | HNLyase_Bact | Homoserine_transacetylase_like_est | Hydroxynitrile_lyase | LYsophospholipase_carboxylesterase | Lipase_3 | Pectinacetylesterase-Notum Acetate | Aryl ester | Chromogen | pNP
Paranitrophenylbutyrate6_AlphaBeta_hydrolase | A85-EsteraseD-FGH | ABHD11-Acetyl_transferase | AlphaBeta_hydrolase | Bacterial_Est97 | Bacterial_EstLip_FamX | Bacterial_esterase | Bacterial_lip_FamI.5 | BioH | Canar_LipB | CarbLipBact_1 | Carb_B_Bacteria | Carb_B_Chordata | Cholesterol_esterase | Cutinase | Duf_1100-S | Duf_3089 | Est9X | Fungal_carboxylesterase_lipase | GTSAGmotif | HNLyase_Bact | Hormone-sensitive_lipase_like | LYsophospholipase_carboxylesterase | Lipase_3 | Pectinacetylesterase-Notum | Polyesterase-lipase-cutinase | abh_upf00227 Butyrate | Chromogen | Water-soluble short chain ester | pNP
Paranitrophenylpropionate6_AlphaBeta_hydrolase | BioH | Fungal_carboxylesterase_lipase | HNLyase_Bact | Hormone-sensitive_lipase_like | Monoglyceridelipase_lysophospholip Chromogen | Propionate | Water-soluble short chain ester | pNP
ParanitrophenylthioacetateS9N_PREPL_Peptidase_S9 Chromogen | pNP
Paranitrophenylvalerate5_AlphaBeta_hydrolase | 6_AlphaBeta_hydrolase | Bacterial_EstLip_FamX | Fungal_carboxylesterase_lipase | Hormone-sensitive_lipase_like Chromogen | Water-soluble short chain ester | pNP
ParaoxonBCHE | Dienelactone_hydrolase Derivative of Paraoxon | Insecticide | Organophosphate | pNP
Z-Gly-Pro-pNAS9N_PPCE_Peptidase_S9 Benzyloxycarbonyl | Carboxamide | Chromogen | Peptide | Pyrrolidine | p-nitroaniline | pNP
butyl-N(para-nitrophenyl)carbamateCutinase Carbamate | Chromogen | Polyurethane | pNP
pNPXBD-FAE Aryl ester | Chromogen | Glycoside | pNP

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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