| Title : New Thermophilic alpha\/beta Class Epoxide Hydrolases Found in Metagenomes From Hot Environments - Ferrandi_2018_Front.Bioeng.Biotechnol_6_144 |
| Author(s) : Ferrandi EE , Sayer C , De Rose SA , Guazzelli E , Marchesi C , Saneei V , Isupov MN , Littlechild JA , Monti D |
| Ref : Front Bioeng Biotechnol , 6 :144 , 2018 |
| Abstract : Two novel epoxide hydrolases (EHs), Sibe-EH and CH65-EH, were identified in the metagenomes of samples collected in hot springs in Russia and China, respectively. The two alpha/beta hydrolase superfamily fold enzymes were cloned, over-expressed in Escherichia coli, purified and characterized. The new EHs were active toward a broad range of substrates, and in particular, Sibe-EH was excellent in the desymmetrization of cis-2,3-epoxybutane producing the (2R,3R)-diol product with ee exceeding 99%. Interestingly these enzymes also hydrolyse (4R)-limonene-1,2-epoxide with Sibe-EH being specific for the trans isomer. The Sibe-EH is a monomer in solution whereas the CH65-EH is a dimer. Both enzymes showed high melting temperatures with the CH65-EH being the highest at 85 degrees C retaining 80% of its initial activity after 3 h thermal treatment at 70 degrees C making it the most thermal tolerant wild type epoxide hydrolase described. The Sibe-EH and CH65-EH have been crystallized and their structures determined to high resolution, 1.6 and 1.4 A, respectively. The CH65-EH enzyme forms a dimer via its cap domains with different relative orientation of the monomers compared to previously described EHs. The entrance to the active site cavity is located in a different position in CH65-EH and Sibe-EH in relation to other known bacterial and mammalian EHs. |
| ESTHER : Ferrandi_2018_Front.Bioeng.Biotechnol_6_144 |
| PubMedSearch : Ferrandi_2018_Front.Bioeng.Biotechnol_6_144 |
| PubMedID: 30386778 |
| Gene_locus related to this paper: 9bact-a0a1u9wz52 , 9bact-a0a1u9wz58 |
| Gene_locus related to this paper: 9bact-a0a1u9wz52 , 9bact-a0a1u9wz58 |
Ferrandi EE, Sayer C, De Rose SA, Guazzelli E, Marchesi C, Saneei V, Isupov MN, Littlechild JA, Monti D (2018)
New Thermophilic alpha\/beta Class Epoxide Hydrolases Found in Metagenomes From Hot Environments
Front Bioeng Biotechnol
6 :144
Ferrandi EE, Sayer C, De Rose SA, Guazzelli E, Marchesi C, Saneei V, Isupov MN, Littlechild JA, Monti D (2018)
Front Bioeng Biotechnol
6 :144