| Title : Biochemical Characterization of a Novel alpha\/beta-Hydrolase\/FSH from the White Shrimp Litopenaeus vannamei - Garcia-Orozco_2019_Biomolecules_9_ |
| Author(s) : Garcia-Orozco KD , Cinco-Moroyoqui F , Angulo-Sanchez LT , Marquez-Rios E , Burgos-Hernandez A , Cardenas-Lopez JL , Gomez-Aguilar C , Corona-Martinez DO , Saab-Rincon G , Sotelo-Mundo RR |
| Ref : Biomolecules , 9 : , 2019 |
| Abstract : (1) Background: Lipases and esterases are important enzymes that share the alpha/beta hydrolase fold. The activity and cellular localization are important characteristics to understand the role of such enzymes in an organism. (2) Methods: Bioinformatic and biochemical tools were used to describe a new alpha/beta hydrolase from a Litopenaeus vannamei transcriptome (LvFHS for Family Serine Hydrolase). (3) Results: The enzyme was obtained by heterologous overexpression in Escherichia coli and showed hydrolytic activity towards short-chain lipid substrates and high affinity to long-chain lipid substrates. Anti-LvFHS antibodies were produced in rabbit that immunodetected the LvFSH enzyme in several shrimp tissues. (4) Conclusions: The protein obtained and analyzed was an alpha/beta hydrolase with esterase and lipase-type activity towards long-chain substrates up to 12 carbons; its immunodetection in shrimp tissues suggests that it has an intracellular localization, and predicted roles in energy mobilization and signal transduction. |
| ESTHER : Garcia-Orozco_2019_Biomolecules_9_ |
| PubMedSearch : Garcia-Orozco_2019_Biomolecules_9_ |
| PubMedID: 31683580 |
| Gene_locus related to this paper: penva-LvFSH |
| Gene_locus related to this paper: penva-LvFSH |
Garcia-Orozco KD, Cinco-Moroyoqui F, Angulo-Sanchez LT, Marquez-Rios E, Burgos-Hernandez A, Cardenas-Lopez JL, Gomez-Aguilar C, Corona-Martinez DO, Saab-Rincon G, Sotelo-Mundo RR (2019)
Biochemical Characterization of a Novel alpha\/beta-Hydrolase\/FSH from the White Shrimp Litopenaeus vannamei
Biomolecules
9 :
Garcia-Orozco KD, Cinco-Moroyoqui F, Angulo-Sanchez LT, Marquez-Rios E, Burgos-Hernandez A, Cardenas-Lopez JL, Gomez-Aguilar C, Corona-Martinez DO, Saab-Rincon G, Sotelo-Mundo RR (2019)
Biomolecules
9 :