| Title : Compositional profile of alpha \/ beta-hydrolase fold proteins in mangrove soil metagenomes: prevalence of epoxide hydrolases and haloalkane dehalogenases in oil-contaminated sites - Jimenez_2015_Microb.Biotechnol_8_604 |
| Author(s) : Jimenez DJ , Dini-Andreote F , Ottoni JR , de Oliveira VM , van Elsas JD , Andreote FD |
| Ref : Microb Biotechnol , 8 :604 , 2015 |
| Abstract : The occurrence of genes encoding biotechnologically relevant alpha/beta-hydrolases in mangrove soil microbial communities was assessed using data obtained by whole-metagenome sequencing of four mangroves areas, denoted BrMgv01 to BrMgv04, in Sao Paulo, Brazil. The sequences (215 Mb in total) were filtered based on local amino acid alignments against the Lipase Engineering Database. In total, 5923 unassembled sequences were affiliated with 30 different alpha/beta-hydrolase fold superfamilies. The most abundant predicted proteins encompassed cytosolic hydrolases (abH08; approximately 23%), microsomal hydrolases (abH09; approximately 12%) and Moraxella lipase-like proteins (abH04 and abH01; < 5%). Detailed analysis of the genes predicted to encode proteins of the abH08 superfamily revealed a high proportion related to epoxide hydrolases and haloalkane dehalogenases in polluted mangroves BrMgv01-02-03. This suggested selection and putative involvement in local degradation/detoxification of the pollutants. Seven sequences that were annotated as genes for putative epoxide hydrolases and five for putative haloalkane dehalogenases were found in a fosmid library generated from BrMgv02 DNA. The latter enzymes were predicted to belong to Actinobacteria, Deinococcus-Thermus, Planctomycetes and Proteobacteria. Our integrated approach thus identified 12 genes (complete and/or partial) that may encode hitherto undescribed enzymes. The low amino acid identity (< 60%) with already-described genes opens perspectives for both production in an expression host and genetic screening of metagenomes. |
| ESTHER : Jimenez_2015_Microb.Biotechnol_8_604 |
| PubMedSearch : Jimenez_2015_Microb.Biotechnol_8_604 |
| PubMedID: 25171437 |
Jimenez DJ, Dini-Andreote F, Ottoni JR, de Oliveira VM, van Elsas JD, Andreote FD (2015)
Compositional profile of alpha \/ beta-hydrolase fold proteins in mangrove soil metagenomes: prevalence of epoxide hydrolases and haloalkane dehalogenases in oil-contaminated sites
Microb Biotechnol
8 :604
Jimenez DJ, Dini-Andreote F, Ottoni JR, de Oliveira VM, van Elsas JD, Andreote FD (2015)
Microb Biotechnol
8 :604