| Title : A new and promiscuous alpha\/beta hydrolase from Acinetobacter tandoii DSM 14970( T) inactivates the mycotoxin ochratoxin A - Sanchez-Arroyo_2024_Appl.Microbiol.Biotechnol_108_230 |
| Author(s) : Sanchez-Arroyo A , Plaza-Vinuesa L , Abeijon-Mukdsi MC , de Las Rivas B , Mancheno JM , Munoz R |
| Ref : Applied Microbiology & Biotechnology , 108 :230 , 2024 |
| Abstract : The presence of ochratoxin A (OTA) in food and feed represents a serious concern since it raises severe health implications. Bacterial strains of the Acinetobacter genus hydrolyse the amide bond of OTA yielding non-toxic OTalpha and L-beta-phenylalanine; in particular, the carboxypeptidase PJ15_1540 from Acinetobacter sp. neg1 has been identified as an OTA-degrading enzyme. Here, we describe the ability to transform OTA of cell-free protein extracts from Acinetobacter tandoii DSM 14970( T), a strain isolated from sludge plants, and also report on the finding of a new and promiscuous alpha/beta hydrolase (ABH), with close homologs highly distributed within the Acinetobacter genus. ABH from A. tandoii (AtABH) exhibited amidase activity against OTA and OTB mycotoxins, as well as against several carboxypeptidase substrates. The predicted structure of AtABH reveals an alpha/beta hydrolase core composed of a parallel, six-stranded beta-sheet, with a large cap domain similar to the marine esterase EprEst. Further biochemical analyses of AtABH reveal that it is an efficient esterase with a similar specificity profile as EprEst. Molecular docking studies rendered a consistent OTA-binding mode. We proposed a potential procedure for preparing new OTA-degrading enzymes starting from promiscuous alpha/beta hydrolases based on our results. KEY POINTS: AtABH is a promiscuous alphabeta hydrolase with both esterase and amidohydrolase activities AtABH hydrolyses the amide bond of ochratoxin A rendering nontoxic OTalpha Promiscuous alphabeta hydrolases are a possible source of new OTA-degrading enzymes. |
| ESTHER : Sanchez-Arroyo_2024_Appl.Microbiol.Biotechnol_108_230 |
| PubMedSearch : Sanchez-Arroyo_2024_Appl.Microbiol.Biotechnol_108_230 |
| PubMedID: 38393350 |
| Gene_locus related to this paper: 9gamm-acitaABH |
| Gene_locus related to this paper: 9gamm-acitaABH |
Sanchez-Arroyo A, Plaza-Vinuesa L, Abeijon-Mukdsi MC, de Las Rivas B, Mancheno JM, Munoz R (2024)
A new and promiscuous alpha\/beta hydrolase from Acinetobacter tandoii DSM 14970( T) inactivates the mycotoxin ochratoxin A
Applied Microbiology & Biotechnology
108 :230
Sanchez-Arroyo A, Plaza-Vinuesa L, Abeijon-Mukdsi MC, de Las Rivas B, Mancheno JM, Munoz R (2024)
Applied Microbiology & Biotechnology
108 :230