| Title : J1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from Bacillus laterosporus J1, is a member of the alpha\/beta-hydrolase fold superfamily - Yau_2006_FEBS.Lett_580_1465 |
| Author(s) : Yau MH , Wang J , Tsang PW , Fong WP |
| Ref : FEBS Letters , 580 :1465 , 2006 |
| Abstract : J1 acylase, a glutaryl-7-aminocephalosporanic acid acylase (GCA) isolated from Bacillus laterosporus J1, has been conventionally grouped as the only member of class V GCA, although its amino acid sequence shares less than 10% identity with members of other classes of GCA. Instead, it shows higher sequence similarities with Rhodococcus sp. strain MB1 cocaine esterase (RhCocE) and Acetobacter turbidans alpha-amino acid ester hydrolase (AtAEH), members of the alpha/beta-hydrolase fold superfamily. Homology modeling and secondary structure prediction indicate that the N-terminal region of J1 acylase has an alpha/beta-hydrolase folding pattern. The catalytic triads in RhCocE and AtAEH were identified in J1 acylase as S125, D264 and H309. Mutations to alanine at these positions were found to completely inactivate the enzyme. These results suggest that J1 acylase is a member of the alpha/beta-hydrolase fold superfamily with a serine-histidine-aspartate catalytic triad. |
| ESTHER : Yau_2006_FEBS.Lett_580_1465 |
| PubMedSearch : Yau_2006_FEBS.Lett_580_1465 |
| PubMedID: 16469317 |
| Gene_locus related to this paper: brela-Q45289 |
| Gene_locus related to this paper: brela-Q45289 |
Yau MH, Wang J, Tsang PW, Fong WP (2006)
J1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from Bacillus laterosporus J1, is a member of the alpha\/beta-hydrolase fold superfamily
FEBS Letters
580 :1465
Yau MH, Wang J, Tsang PW, Fong WP (2006)
FEBS Letters
580 :1465