Title : Cloning and expression of the PHA synthase gene from a locally isolated Chromobacterium sp. USM2 - Zhao_2023_Food.Chem_439_138100 |
Author(s) : Bhubalan K , Kam YC , Yong KH , Sudesh K |
Ref : Mal J Microbiol , 6 :1 , 2010 |
Abstract :
Chromobacterium sp. USM2, a locally isolated bacterium was found to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV) copolymer with high 3HV monomer composition. The PHA synthase gene was cloned and expressed in Cupriavidus necator PHB4 to investigate the possibilities of incorporating other monomer. The recombinant successfully incorporated 3-hydroxyhexanoate (3HHx) monomer when fed with crude palm kernel oil (CPKO) as the sole carbon source. Approximately 63 2 wt% of P(3HB-co-3HHx) copolymer with 4 mol% of 3HHx was synthesized from 5 g/L of oil after 48 h of cultivation. In addition, P(3HB-co-3HV-co-3HHx) terpolymer with 9 mol% 3HV and 4 mol% 3HHx could be synthesized with a mixture of CPKO and sodium valerate. The presence of 3HV and 3HHx monomers in the copolymer and terpolymer was further confirmed with +H-NMR analysis. This locally isolated PHA synthase has demonstrated its ability to synthesize P(3HB-co-3HHx) copolymer from a readily available and renewable carbon source; CPKO, without the addition of 3HHx precursors. |
PubMedSearch : Zhao_2023_Food.Chem_439_138100 |
PubMedID: |
Gene_locus related to this paper: 9neis-e1apk1 |
Gene_locus | 9neis-e1apk1 |
Bhubalan K, Kam YC, Yong KH, Sudesh K (2010)
Cloning and expression of the PHA synthase gene from a locally isolated Chromobacterium sp. USM2
Mal J Microbiol
6 :1
Bhubalan K, Kam YC, Yong KH, Sudesh K (2010)
Mal J Microbiol
6 :1