longtext: 1BN6-pdb

content
HEADER    HYDROLASE                               31-JUL-98   1BN6
TITLE     HALOALKANE DEHALOGENASE FROM A RHODOCOCCUS SPECIES
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: HALOALKANE DEHALOGENASE;
COMPND   3 CHAIN: A;
COMPND   4 EC: 3.8.1.5;
COMPND   5 ENGINEERED: YES;
COMPND   6 BIOLOGICAL_UNIT: MONOMER
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: RHODOCOCCUS SP.;
SOURCE   3 COLLECTION: ATCC 55388;
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI
KEYWDS    DEHALOGENASE, ALPHA/BETA-HYDROLASE, DHLA, CRYSTAL STRUCTURE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    J.NEWMAN,T.S.PEAT,R.RICHARD,L.KAN,P.E.SWANSON,J.A.AFFHOLTER,
AUTHOR   2 I.H.HOLMES,J.F.SCHINDLER,C.J.UNKEFER,T.C.TERWILLIGER
REVDAT   1   18-FEB-00 1BN6    0
JRNL        AUTH   J.NEWMAN,T.S.PEAT,R.RICHARD,L.KAN,P.E.SWANSON,
JRNL        AUTH 2 J.A.AFFHOLTER,I.H.HOLMES,J.F.SCHINDLER,C.J.UNKEFER,
JRNL        AUTH 3 T.C.TERWILLIGER
JRNL        TITL   HALOALKANE DEHALOGENASES: STRUCTURE OF A
JRNL        TITL 2 RHODOCOCCUS ENZYME
JRNL        REF    BIOCHEMISTRY                  V.  38 16105 1999
JRNL        REFN   ASTM BICHAW  US ISSN 0006-2960
REMARK   1
REMARK   2
REMARK   2 RESOLUTION. 1.5  ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : CNS 0.3
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,
REMARK   3                 GROSSE-KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,
REMARK   3                 PANNU,READ,RICE,SIMONSON,WARREN
REMARK   3
REMARK   3  REFINEMENT TARGET : MLF
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.5
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 50.0
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 3.0
REMARK   3   OUTLIER CUTOFF HIGH (RMS(ABS(F))) : NULL
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 96.8
REMARK   3   NUMBER OF REFLECTIONS             : 51084
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE            (WORKING SET) : 0.167
REMARK   3   FREE R VALUE                     : 0.174
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5
REMARK   3   FREE R VALUE TEST SET COUNT      : 2577
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : 0.003
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 6
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.50
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.59
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 96.0
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : 7873
REMARK   3   BIN R VALUE           (WORKING SET) : 0.179
REMARK   3   BIN FREE R VALUE                    : 0.202
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : 5.3
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : 437
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : 0.010
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 2336
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 4
REMARK   3   SOLVENT ATOMS            : 329
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 11.5
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 10.4
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.413
REMARK   3    B22 (A**2) : -0.224
REMARK   3    B33 (A**2) : -0.189
REMARK   3    B12 (A**2) : 0.0
REMARK   3    B13 (A**2) : 0.0
REMARK   3    B23 (A**2) : 0.0
REMARK   3
REMARK   3  ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM LUZZATI PLOT        (A) : 0.14
REMARK   3   ESD FROM SIGMAA              (A) : 0.05
REMARK   3   LOW RESOLUTION CUTOFF        (A) : 5.0
REMARK   3
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : 0.14
REMARK   3   ESD FROM C-V SIGMAA          (A) : 0.09
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.
REMARK   3   BOND LENGTHS                 (A) : 0.033
REMARK   3   BOND ANGLES            (DEGREES) : 2.6
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 23.6
REMARK   3   IMPROPER ANGLES        (DEGREES) : 2.17
REMARK   3
REMARK   3  ISOTROPIC THERMAL MODEL : RESTRAINED
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA
REMARK   3   MAIN-CHAIN BOND              (A**2) : 0.41  ; 1.50
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : 0.69  ; 2.0
REMARK   3   SIDE-CHAIN BOND              (A**2) : 0.50  ; 2.0
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : 0.79  ; 2.5
REMARK   3
REMARK   3  BULK SOLVENT MODELING.
REMARK   3   METHOD USED : NULL
REMARK   3   KSOL        : 0.385
REMARK   3   BSOL        : 28.53
REMARK   3
REMARK   3  NCS MODEL : NULL
REMARK   3
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM
REMARK   3  PARAMETER FILE  2  : WATER_REP.PARAM
REMARK   3  TOPOLOGY FILE  1   : PROTEIN.TOP
REMARK   3  TOPOLOGY FILE  2   : WATER.TOP
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   3   CUTOFF HIGH (ABS(F)) : 1447832.12
REMARK   4
REMARK   4 1BN6 COMPLIES WITH FORMAT V. 2.3, 09-JULY-1998
REMARK   6
REMARK   6 DID NOT SEE ANY OF THE C-TERMINAL EXFLAG TAG
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : APR-1998
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 7.0
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : NSLS
REMARK 200  BEAMLINE                       : X8C
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.8157
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MOSFLM
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 53599
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.48
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.0
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.2
REMARK 200  DATA REDUNDANCY                : 4.8
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : 0.065
REMARK 200   FOR THE DATA SET  : 9.0
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.48
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.56
REMARK 200  COMPLETENESS FOR SHELL     (%) : 83.6
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.0
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : 0.264
REMARK 200   FOR SHELL         : 2.8
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: NULL
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR
REMARK 200    REPLACEMENT
REMARK 200 SOFTWARE USED: CNS
REMARK 200 STARTING MODEL: MODEL AT PH 5.5
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 38.8
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.43
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS:
REMARK 280 1 UL PROTEIN (5MG/ML PROTEIN IN 150MM AMMONIUM SULFATE,
REMARK 280 10MM TRISSO4 PH 7.5, 1MM EDTA) +1 UL RESERVOIR OVER
REMARK 280 20-25% PEG 1.5K, 0.2M NAACETATE, 0.1M MES PH 5.5 AT 8C
REMARK 280 SOAKED INTO SAME CONDITIONS BUT HEPES PH 7.0 BEFORE DATA
REMARK 280 COLLECTION
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z
REMARK 290       3555   1/2-X,1/2+Y,-Z
REMARK 290       4555   1/2+X,1/2-Y,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       46.75956
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       39.98401
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       46.75956
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       39.98401
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A    12
REMARK 465     SER A    13
REMARK 465     GLU A    14
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   O    HOH     559     O    HOH     523     3547     1.75
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS
REMARK 500
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.
REMARK 500                                 MODEL     OMEGA
REMARK 500 ASP    167    VAL    168          0       117.23
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 15 DEGREES (M=MODEL NUMBER; RES=RESIDUE NAME;
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION
REMARK 500 CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500  0 GLU   109   CB  -  CG  -  CD  ANGL. DEV. =  24.9 DEGREES
REMARK 500  0 ALA   166   N   -  CA  -  CB  ANGL. DEV. =  39.7 DEGREES
REMARK 500  0 ALA   166   C   -  CA  -  CB  ANGL. DEV. =  17.7 DEGREES
REMARK 500  0 ALA   166   N   -  CA  -  C   ANGL. DEV. =  15.5 DEGREES
REMARK 500  0 ALA   166   C-1 -  N   -  CA  ANGL. DEV. =  19.8 DEGREES
REMARK 500  0 ASP   167   C   -  CA  -  CB  ANGL. DEV. =  35.8 DEGREES
REMARK 500  0 ASP   167   CA  -  CB  -  CG  ANGL. DEV. =  17.0 DEGREES
REMARK 500  0 ASP   167  CA-1 -  C-1 -  N   ANGL. DEV. =  27.8 DEGREES
REMARK 500  0 ASP   167   O-1 -  C-1 -  N   ANGL. DEV. =  27.7 DEGREES
REMARK 500  0 VAL   168  CA-1 -  C-1 -  N   ANGL. DEV. =  29.8 DEGREES
REMARK 500  0 VAL   168   O-1 -  C-1 -  N   ANGL. DEV. =  33.9 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 4*RMSD AND BY MORE THAN 0.150 ANGSTROMS (M=MODEL
REMARK 500 NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 500 NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,1X,A4,A1,2X,
REMARK 500 A3,1X,A1,I4,A1,1X,A4,A1,4X,F5.3)
REMARK 500
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500  0 LYS A 128   CB B  LYS A 128   CA      0.207
REMARK 500  0 GLU A 158   CB    GLU A 158   CG      0.153
REMARK 500  0 ALA A 166   N  A  ALA A 166   CA A    0.913
REMARK 500  0 ALA A 166   CA A  ALA A 166   C  A    0.203
REMARK 500  0 ALA A 166   CB A  ALA A 166   CA A    0.450
REMARK 500  0 ASP A 167   N  A  ASP A 167   CA A    0.267
REMARK 500  0 ASP A 167   N  B  ASP A 167   CA B    0.185
REMARK 500  0 ASP A 167   CA B  ASP A 167   C  B    0.160
REMARK 500  0 VAL A 168   N     VAL A 168   CA      0.210
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: CAT
REMARK 800 SITE_DESCRIPTION: CATALYTIC TRIAD RESIDUES
REMARK 999
REMARK 999 SEQUENCE
REMARK 999 FIRST 11 RESIDUES ARE A HIS-TAG, THE LAST 12 ARE AN EXFLAG.
DBREF  1BN6 A   12   304  GB     3114657  AF060871         1    293
SEQADV 1BN6 VAL A  183  GB   3114657   ALA A 172 CONFLICT
SEQADV 1BN6 ILE A  220  GB   3114657   LEU A 209 CONFLICT
SEQADV 1BN6 GLY A  303  GB   3114657   ALA A 292 CONFLICT
SEQADV 1BN6 ALA A  305  GB   3114657             CONFLICT
SEQRES   1 A  294  MET SER GLU ILE GLY THR GLY PHE PRO PHE ASP PRO HIS
SEQRES   2 A  294  TYR VAL GLU VAL LEU GLY GLU ARG MET HIS TYR VAL ASP
SEQRES   3 A  294  VAL GLY PRO ARG ASP GLY THR PRO VAL LEU PHE LEU HIS
SEQRES   4 A  294  GLY ASN PRO THR SER SER TYR LEU TRP ARG ASN ILE ILE
SEQRES   5 A  294  PRO HIS VAL ALA PRO SER HIS ARG CYS ILE ALA PRO ASP
SEQRES   6 A  294  LEU ILE GLY MET GLY LYS SER ASP LYS PRO ASP LEU ASP
SEQRES   7 A  294  TYR PHE PHE ASP ASP HIS VAL ARG TYR LEU ASP ALA PHE
SEQRES   8 A  294  ILE GLU ALA LEU GLY LEU GLU GLU VAL VAL LEU VAL ILE
SEQRES   9 A  294  HIS ASP TRP GLY SER ALA LEU GLY PHE HIS TRP ALA LYS
SEQRES  10 A  294  ARG ASN PRO GLU ARG VAL LYS GLY ILE ALA CYS MET GLU
SEQRES  11 A  294  PHE ILE ARG PRO ILE PRO THR TRP ASP GLU TRP PRO GLU
SEQRES  12 A  294  PHE ALA ARG GLU THR PHE GLN ALA PHE ARG THR ALA ASP
SEQRES  13 A  294  VAL GLY ARG GLU LEU ILE ILE ASP GLN ASN ALA PHE ILE
SEQRES  14 A  294  GLU GLY VAL LEU PRO LYS CYS VAL VAL ARG PRO LEU THR
SEQRES  15 A  294  GLU VAL GLU MET ASP HIS TYR ARG GLU PRO PHE LEU LYS
SEQRES  16 A  294  PRO VAL ASP ARG GLU PRO LEU TRP ARG PHE PRO ASN GLU
SEQRES  17 A  294  ILE PRO ILE ALA GLY GLU PRO ALA ASN ILE VAL ALA LEU
SEQRES  18 A  294  VAL GLU ALA TYR MET ASN TRP LEU HIS GLN SER PRO VAL
SEQRES  19 A  294  PRO LYS LEU LEU PHE TRP GLY THR PRO GLY VAL LEU ILE
SEQRES  20 A  294  PRO PRO ALA GLU ALA ALA ARG LEU ALA GLU SER LEU PRO
SEQRES  21 A  294  ASN CYS LYS THR VAL ASP ILE GLY PRO GLY LEU HIS TYR
SEQRES  22 A  294  LEU GLN GLU ASP ASN PRO ASP LEU ILE GLY SER GLU ILE
SEQRES  23 A  294  ALA ARG TRP LEU PRO GLY LEU ALA
FORMUL   2  HOH   *329(H2 O1)
HELIX    1   1 SER A   56  VAL A   66  5                                  11
HELIX    2   2 PHE A   92  ALA A  105  1                                  14
HELIX    3   3 ASP A  117  ARG A  129  1                                  13
HELIX    4   4 PRO A  131  ARG A  133  5                                   3
HELIX    5   5 TRP A  149  GLU A  151  5                                   3
HELIX    6   6 GLU A  154  PHE A  163  1                                  10
HELIX    7   7 VAL A  168  ILE A  173  1                                   6
HELIX    8   8 ALA A  178  GLU A  181  1                                   4
HELIX    9   9 VAL A  183  CYS A  187  1                                   5
HELIX   10  10 GLU A  194  PHE A  204  1                                  11
HELIX   11  11 PRO A  207  GLU A  219  5                                  13
HELIX   12  12 ALA A  227  GLN A  242  1                                  16
HELIX   13  13 PRO A  260  SER A  269  1                                  10
HELIX   14  14 LEU A  285  GLU A  287  5                                   3
HELIX   15  15 PRO A  290  GLY A  303  1                                  14
SHEET    1   A 3 HIS A  24  VAL A  28  0
SHEET    2   A 3 GLU A  31  VAL A  38 -1  N  TYR A  35   O  HIS A  24
SHEET    3   A 3 CYS A  72  PRO A  75 -1  N  ALA A  74   O  VAL A  36
SHEET    1   B 5 PRO A  45  LEU A  49  0
SHEET    2   B 5 VAL A 111  HIS A 116  1  N  VAL A 112   O  PRO A  45
SHEET    3   B 5 VAL A 134  MET A 140  1  N  LYS A 135   O  VAL A 111
SHEET    4   B 5 PRO A 246  TRP A 251  1  N  PRO A 246   O  ILE A 137
SHEET    5   B 5 CYS A 273  ASP A 277  1  N  LYS A 274   O  LYS A 247
CISPEP   1 ASN A   52    PRO A   53          0         0.15
CISPEP   2 GLU A  225    PRO A  226          0        -1.27
CISPEP   3 THR A  253    PRO A  254          0         1.10
SITE     1 CAT  3 ASP A 117  GLU A 141  HIS A 283
CRYST1   93.520   79.970   43.160  90.00  90.00  90.00 P 21 21 2     4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.010693  0.000000  0.000000        0.00000
SCALE2      0.000000  0.012505  0.000000        0.00000
SCALE3      0.000000  0.000000  0.023170        0.00000
END